Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11686 | 5' | -48.8 | NC_003102.1 | + | 82040 | 0.66 | 0.998021 |
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Target: 5'- -gUGGGACuGUCGAUGGU-GCGUCAu- -3' miRNA: 3'- ugAUUCUG-CGGCUACUAuCGUAGUcu -5' |
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| 11686 | 5' | -48.8 | NC_003102.1 | + | 74777 | 0.67 | 0.994546 |
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Target: 5'- cACUucGGAUGCCGAauuUGAaauGCAUCGGu -3' miRNA: 3'- -UGAu-UCUGCGGCU---ACUau-CGUAGUCu -5' |
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| 11686 | 5' | -48.8 | NC_003102.1 | + | 4955 | 0.68 | 0.98837 |
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Target: 5'- uACUAccgauGACGCUGAUGAUucgGGCGuggcggccaccacaUCAGAc -3' miRNA: 3'- -UGAUu----CUGCGGCUACUA---UCGU--------------AGUCU- -5' |
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| 11686 | 5' | -48.8 | NC_003102.1 | + | 15763 | 1.09 | 0.011583 |
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Target: 5'- cACUAAGACGCCGAUGAUAGCAUCAGAc -3' miRNA: 3'- -UGAUUCUGCGGCUACUAUCGUAGUCU- -5' |
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| 11686 | 5' | -48.8 | NC_003102.1 | + | 75730 | 0.74 | 0.867257 |
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Target: 5'- --cGAGACGgacaucgaaaCCGAUGAUGGUGUCGGGa -3' miRNA: 3'- ugaUUCUGC----------GGCUACUAUCGUAGUCU- -5' |
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| 11686 | 5' | -48.8 | NC_003102.1 | + | 20885 | 0.69 | 0.98362 |
|
Target: 5'- cUUGGGGCGgCGGUGGcAGCAuuUCGGAc -3' miRNA: 3'- uGAUUCUGCgGCUACUaUCGU--AGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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