Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11693 | 5' | -52 | NC_003102.1 | + | 6715 | 0.71 | 0.839926 |
Target: 5'- cCGCUgccgcCGGUGUUGGCgcUGCCGAg -3' miRNA: 3'- -GCGAauuu-GCCGCAGCCGa-AUGGCUg -5' |
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11693 | 5' | -52 | NC_003102.1 | + | 129117 | 0.72 | 0.80479 |
Target: 5'- aGCUgccGGCGGgGUagCGGCggcgGCCGACg -3' miRNA: 3'- gCGAau-UUGCCgCA--GCCGaa--UGGCUG- -5' |
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11693 | 5' | -52 | NC_003102.1 | + | 32879 | 0.73 | 0.776667 |
Target: 5'- aGCgUGAACGGCGaCGGCgaACCG-Ca -3' miRNA: 3'- gCGaAUUUGCCGCaGCCGaaUGGCuG- -5' |
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11693 | 5' | -52 | NC_003102.1 | + | 46219 | 0.73 | 0.767005 |
Target: 5'- cCGCgagaacgAAGCGGCuccGUCGGUaaGCCGACg -3' miRNA: 3'- -GCGaa-----UUUGCCG---CAGCCGaaUGGCUG- -5' |
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11693 | 5' | -52 | NC_003102.1 | + | 46033 | 1.11 | 0.00471 |
Target: 5'- cCGCUUAAACGGCGUCGGCUUACCGACg -3' miRNA: 3'- -GCGAAUUUGCCGCAGCCGAAUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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