Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11695 | 3' | -51.3 | NC_003102.1 | + | 65254 | 0.67 | 0.982802 |
Target: 5'- cGGGccgAAGCGGAGGaGGCGGcgauaGACAg -3' miRNA: 3'- aCCU---UUUGCCUCCgUUGCCuag--CUGU- -5' |
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11695 | 3' | -51.3 | NC_003102.1 | + | 87420 | 0.66 | 0.987145 |
Target: 5'- uUGGAAauGAUGGAagaucgaucgacgucGGCGACGGcggUGGCAc -3' miRNA: 3'- -ACCUU--UUGCCU---------------CCGUUGCCua-GCUGU- -5' |
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11695 | 3' | -51.3 | NC_003102.1 | + | 14797 | 0.66 | 0.99084 |
Target: 5'- ---cGAGCGGcGGCAGCGGuuUCGAa- -3' miRNA: 3'- accuUUUGCCuCCGUUGCCu-AGCUgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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