miRNA: 3'- aCCUU--------UUGCCU--CC-GUUGCCUAGCUGU- -5'
miRNA: 3'- -ACCuUUUGCCUCCGUUGCC-UAGcugu -5'
miRNA: 3'- -ACCUUUUGCCUCCGUUGCCUAGCUGU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11695 | 3' | -51.3 | NC_003102.1 | + | 126272 | 0.75 | 0.691575 |
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Target: 5'- cGGGAcggucagcAGCGGGacGGuCAAUGGAUCGACAa -3' miRNA: 3'- aCCUU--------UUGCCU--CC-GUUGCCUAGCUGU- -5' |
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| 11695 | 3' | -51.3 | NC_003102.1 | + | 57051 | 0.76 | 0.617618 |
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Target: 5'- gUGG-AGGCGGAGGCGACGGcAUCa--- -3' miRNA: 3'- -ACCuUUUGCCUCCGUUGCC-UAGcugu -5' |
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| 11695 | 3' | -51.3 | NC_003102.1 | + | 74712 | 1.09 | 0.007551 |
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Target: 5'- gUGGAAAACGGAGGCAACGGAUCGACAc -3' miRNA: 3'- -ACCUUUUGCCUCCGUUGCCUAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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