Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11851 | 3' | -65.1 | NC_003266.2 | + | 15764 | 0.66 | 0.184395 |
Target: 5'- gCGAGCCUugCUGCGCagggccaGGCGcacGGGacgcagGGCCAu -3' miRNA: 3'- -GCUCGGG--GACGCG-------CCGC---UCCa-----CCGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 33785 | 0.66 | 0.180093 |
Target: 5'- gGGGUCCCUGCGCugcauggcgGGCGGgacGGUacuuGCCAu -3' miRNA: 3'- gCUCGGGGACGCG---------CCGCU---CCAc---CGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 13045 | 0.66 | 0.171303 |
Target: 5'- uGGGCCCauguugCgcgcggucaugucgaGCGCGGCGcuggGGGUGGCCc -3' miRNA: 3'- gCUCGGG------Ga--------------CGCGCCGC----UCCACCGGu -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 23052 | 0.66 | 0.170851 |
Target: 5'- uGGGCggCUGCuGCGGCGGcGGUGGUUg -3' miRNA: 3'- gCUCGggGACG-CGCCGCU-CCACCGGu -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 14129 | 0.66 | 0.170851 |
Target: 5'- aCGAGCgCU--CGCGGUGGGGcGGUCAg -3' miRNA: 3'- -GCUCGgGGacGCGCCGCUCCaCCGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 8095 | 0.67 | 0.16639 |
Target: 5'- aCGAGCCC--GCGCGG-GAGGcaGGuCCAg -3' miRNA: 3'- -GCUCGGGgaCGCGCCgCUCCa-CC-GGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 24524 | 0.68 | 0.141764 |
Target: 5'- aCGuGCugcacaccaCCCUGCGCGGgGAGGcccGCCGc -3' miRNA: 3'- -GCuCG---------GGGACGCGCCgCUCCac-CGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 17995 | 0.68 | 0.134325 |
Target: 5'- -aAGCCCCUGcCGcCGGUGGccGUGGCCc -3' miRNA: 3'- gcUCGGGGAC-GC-GCCGCUc-CACCGGu -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 9818 | 0.68 | 0.132164 |
Target: 5'- aCGAGCgCCaggucagcgacgacGCGCucGGCGAGGaUGGCCu -3' miRNA: 3'- -GCUCGgGGa-------------CGCG--CCGCUCC-ACCGGu -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 4450 | 0.68 | 0.127247 |
Target: 5'- uGGGCCCgUGgGCGGCGGcuUGGgCAa -3' miRNA: 3'- gCUCGGGgACgCGCCGCUccACCgGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 23190 | 0.69 | 0.105111 |
Target: 5'- aCGGGCUCUcGCGgccCGGCG-GGUGGCUg -3' miRNA: 3'- -GCUCGGGGaCGC---GCCGCuCCACCGGu -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 26413 | 0.69 | 0.10226 |
Target: 5'- gGAGUagUCCUGgGCGGCGccGGcGGCCAg -3' miRNA: 3'- gCUCG--GGGACgCGCCGCu-CCaCCGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 18077 | 0.71 | 0.077506 |
Target: 5'- gCGAGCCCCcgGuCGCGcGCGGGccacGGCCAc -3' miRNA: 3'- -GCUCGGGGa-C-GCGC-CGCUCca--CCGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 11151 | 0.72 | 0.060053 |
Target: 5'- aCGAGCugacggggaucagCCCUGCGCGcGCGcacGUGGCCGc -3' miRNA: 3'- -GCUCG-------------GGGACGCGC-CGCuc-CACCGGU- -5' |
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11851 | 3' | -65.1 | NC_003266.2 | + | 6353 | 1.07 | 8e-05 |
Target: 5'- aCGAGCCCCUGCGCGGCGAGGUGGCCAc -3' miRNA: 3'- -GCUCGGGGACGCGCCGCUCCACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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