Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11852 | 3' | -53.5 | NC_003266.2 | + | 20991 | 0.72 | 0.368742 |
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Target: 5'- cCGUCUACCuGCGCacccccuuCUCAGCCg -3' miRNA: 3'- -GCAGAUGG-CGCGgcuuuuu-GGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 11149 | 0.72 | 0.365154 |
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Target: 5'- uGUCcagGCCGCGCCGcAGcuCCC-GCCc -3' miRNA: 3'- gCAGa--UGGCGCGGCuUUuuGGGuCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 14894 | 0.74 | 0.283449 |
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Target: 5'- cCGcCUACCGCaGCUGGu--ACCUGGCCu -3' miRNA: 3'- -GCaGAUGGCG-CGGCUuuuUGGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 15676 | 0.74 | 0.276125 |
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Target: 5'- uGUCcgACgCGCGCCGGuacGCCCGcGCCa -3' miRNA: 3'- gCAGa-UG-GCGCGGCUuuuUGGGU-CGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 11505 | 0.75 | 0.248338 |
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Target: 5'- gCGggCUGCCGCuGuCCGAGAAGCUggCGGCCa -3' miRNA: 3'- -GCa-GAUGGCG-C-GGCUUUUUGG--GUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 21336 | 0.75 | 0.229047 |
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Target: 5'- --aCUGCUGCGCCGcAGcGCCCuGGCCa -3' miRNA: 3'- gcaGAUGGCGCGGCuUUuUGGG-UCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 7371 | 0.76 | 0.222904 |
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Target: 5'- gGUCaucaacaACCGgGCCGAGGugcCCCAGCCu -3' miRNA: 3'- gCAGa------UGGCgCGGCUUUuu-GGGUCGG- -5' |
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| 11852 | 3' | -53.5 | NC_003266.2 | + | 6761 | 1.13 | 0.000513 |
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Target: 5'- uCGUCUACCGCGCCGAAAAACCCAGCCg -3' miRNA: 3'- -GCAGAUGGCGCGGCUUUUUGGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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