Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11859 | 5' | -57.4 | NC_003266.2 | + | 26555 | 0.68 | 0.348648 |
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Target: 5'- gACCguACUACUUCCgcgagacgcccagGCCgAAGUCCAGCu -3' miRNA: 3'- gUGG--UGGUGAGGG-------------CGGgUUCAGGUUG- -5' |
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| 11859 | 5' | -57.4 | NC_003266.2 | + | 27150 | 0.66 | 0.449369 |
|
Target: 5'- -cCCACCuGCUUCguaucuucagccagCGCCCGauccuGGUCCAGCg -3' miRNA: 3'- guGGUGG-UGAGG--------------GCGGGU-----UCAGGUUG- -5' |
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| 11859 | 5' | -57.4 | NC_003266.2 | + | 30776 | 0.67 | 0.384941 |
|
Target: 5'- aACgCACCcgaggcgcucugGCUCCCGCCUGAucCCGACa -3' miRNA: 3'- gUG-GUGG------------UGAGGGCGGGUUcaGGUUG- -5' |
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| 11859 | 5' | -57.4 | NC_003266.2 | + | 30848 | 0.67 | 0.422677 |
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Target: 5'- gCACCACCAC-CacaGCCUAGGccCCAAUa -3' miRNA: 3'- -GUGGUGGUGaGgg-CGGGUUCa-GGUUG- -5' |
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| 11859 | 5' | -57.4 | NC_003266.2 | + | 33338 | 0.67 | 0.422677 |
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Target: 5'- aGCUGCUACUCaagggGUCCGGGUCCAGg -3' miRNA: 3'- gUGGUGGUGAGgg---CGGGUUCAGGUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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