Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11900 | 3' | -56.6 | NC_003266.2 | + | 6382 | 0.69 | 0.347442 |
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Target: 5'- -cGUCGGGGGgGUCAGCAuCGAuGGUg -3' miRNA: 3'- acUAGCUCCUgCAGUCGUcGCUcCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 8455 | 0.69 | 0.347442 |
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Target: 5'- gUGGUggcaGGGGcACGUCGGCGccgcGCGcGGGCa -3' miRNA: 3'- -ACUAg---CUCC-UGCAGUCGU----CGCuCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 24944 | 0.7 | 0.322604 |
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Target: 5'- --cUCGcGGAagGUCAGCGGCacgaaguccGAGGGCa -3' miRNA: 3'- acuAGCuCCUg-CAGUCGUCG---------CUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 22503 | 0.7 | 0.299116 |
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Target: 5'- aUGAUCGAgauGGAUGU-GGCaAGCGAgaacGGGCa -3' miRNA: 3'- -ACUAGCU---CCUGCAgUCG-UCGCU----CCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 15012 | 0.73 | 0.206898 |
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Target: 5'- ---cCGAGGugGUgAGCAGCGuccAGGaGCg -3' miRNA: 3'- acuaGCUCCugCAgUCGUCGC---UCC-CG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 25128 | 0.73 | 0.201352 |
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Target: 5'- aUGAUCuGGGucuCGcUCAGCAGCuGGGGGUu -3' miRNA: 3'- -ACUAGcUCCu--GC-AGUCGUCG-CUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 15686 | 0.77 | 0.099507 |
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Target: 5'- cGGUgGAgacGGGCG-CGGCGGCGGGGGUg -3' miRNA: 3'- aCUAgCU---CCUGCaGUCGUCGCUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 24978 | 1.11 | 0.000327 |
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Target: 5'- gUGAUCGAGGACGUCAGCAGCGAGGGCc -3' miRNA: 3'- -ACUAGCUCCUGCAGUCGUCGCUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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