Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11900 | 3' | -56.6 | NC_003266.2 | + | 23960 | 0.66 | 0.522637 |
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Target: 5'- aGAucuUCGAGGGucUgGGCAGCGAcgagacucGGGCc -3' miRNA: 3'- aCU---AGCUCCUgcAgUCGUCGCU--------CCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 24944 | 0.7 | 0.322604 |
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Target: 5'- --cUCGcGGAagGUCAGCGGCacgaaguccGAGGGCa -3' miRNA: 3'- acuAGCuCCUg-CAGUCGUCG---------CUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 24978 | 1.11 | 0.000327 |
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Target: 5'- gUGAUCGAGGACGUCAGCAGCGAGGGCc -3' miRNA: 3'- -ACUAGCUCCUGCAGUCGUCGCUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 25128 | 0.73 | 0.201352 |
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Target: 5'- aUGAUCuGGGucuCGcUCAGCAGCuGGGGGUu -3' miRNA: 3'- -ACUAGcUCCu--GC-AGUCGUCG-CUCCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 25659 | 0.67 | 0.469957 |
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Target: 5'- ---cCGAGGACGaCGGUcuGGCGGcGGCg -3' miRNA: 3'- acuaGCUCCUGCaGUCG--UCGCUcCCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 26533 | 0.68 | 0.382635 |
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Target: 5'- gUGAUUGcGGGGCGU-GGCGGUGAGcGCu -3' miRNA: 3'- -ACUAGC-UCCUGCAgUCGUCGCUCcCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 30073 | 0.67 | 0.449649 |
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Target: 5'- gUGAUCGGGGugGUCAuccUAGCGAu-GCu -3' miRNA: 3'- -ACUAGCUCCugCAGUc--GUCGCUccCG- -5' |
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| 11900 | 3' | -56.6 | NC_003266.2 | + | 33409 | 0.66 | 0.522637 |
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Target: 5'- cUGGUaCGGcGGGCG-CAGCAGCGGauGCg -3' miRNA: 3'- -ACUA-GCU-CCUGCaGUCGUCGCUccCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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