Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11914 | 3' | -61.1 | NC_003278.1 | + | 21322 | 0.66 | 0.289809 |
Target: 5'- cUGcuCGCCGGcuuagggcagaucaaGCGCGccGUCCAGCGCg -3' miRNA: 3'- aACucGCGGUC---------------CGCGCc-UAGGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 9389 | 0.66 | 0.286889 |
Target: 5'- ---uGCGCCAGGUGuuGua-CAGCGCc -3' miRNA: 3'- aacuCGCGGUCCGCgcCuagGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 3462 | 0.66 | 0.272639 |
Target: 5'- --aGGUGCCGGcCGCGGcucgCCAGaCGCg -3' miRNA: 3'- aacUCGCGGUCcGCGCCua--GGUC-GCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 15895 | 0.66 | 0.272639 |
Target: 5'- gUUGAugGCGCCAuuGGcCGCcuGGAUCUGcGCGCg -3' miRNA: 3'- -AACU--CGCGGU--CC-GCG--CCUAGGU-CGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 16132 | 0.66 | 0.272639 |
Target: 5'- ---cGUGCCuguuGCGCGG--CCAGCGCg -3' miRNA: 3'- aacuCGCGGuc--CGCGCCuaGGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 24826 | 0.66 | 0.26573 |
Target: 5'- cUGGGCGUCGcccuuGGCGCGcagaUAGCGCa -3' miRNA: 3'- aACUCGCGGU-----CCGCGCcuagGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 1888 | 0.66 | 0.26573 |
Target: 5'- -aGGGCGCUcuguaGGGCGCaGAaccacUCCGGCa- -3' miRNA: 3'- aaCUCGCGG-----UCCGCGcCU-----AGGUCGcg -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 15223 | 0.66 | 0.261653 |
Target: 5'- -aGAGCGCaucgAGGUGCcggccaugcugcccuGGAcgCCAGCGUu -3' miRNA: 3'- aaCUCGCGg---UCCGCG---------------CCUa-GGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 19603 | 0.67 | 0.252337 |
Target: 5'- aUGAGCGCgAccuGcGCGCGcaGAUCCAG-GCg -3' miRNA: 3'- aACUCGCGgU---C-CGCGC--CUAGGUCgCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 8675 | 0.67 | 0.251682 |
Target: 5'- --cGGCGCCgccaccgAGGaCGCGGugcgugccuUCCAGCGUu -3' miRNA: 3'- aacUCGCGG-------UCC-GCGCCu--------AGGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 26526 | 0.67 | 0.233296 |
Target: 5'- ---cGC-CCGGGCGaGGGUucaCCAGCGCg -3' miRNA: 3'- aacuCGcGGUCCGCgCCUA---GGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 30331 | 0.67 | 0.233296 |
Target: 5'- -gGAGCGCCAGGaCGCcGucaacgCCGacGCGCc -3' miRNA: 3'- aaCUCGCGGUCC-GCGcCua----GGU--CGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 13852 | 0.67 | 0.227223 |
Target: 5'- -gGGGCGCU-GGCGCcGAgcaCCAGgGCa -3' miRNA: 3'- aaCUCGCGGuCCGCGcCUa--GGUCgCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 2453 | 0.67 | 0.227223 |
Target: 5'- ---uGUGCCAGGUGCc-AUCCAGCGa -3' miRNA: 3'- aacuCGCGGUCCGCGccUAGGUCGCg -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 29201 | 0.67 | 0.221284 |
Target: 5'- --cAGUGcCCAGGuCGCGGcggCCAGCGa -3' miRNA: 3'- aacUCGC-GGUCC-GCGCCua-GGUCGCg -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 21470 | 0.68 | 0.209803 |
Target: 5'- -cGAucGuCGCCAGGCcgugGCGGGccaggaugUCCGGCGUg -3' miRNA: 3'- aaCU--C-GCGGUCCG----CGCCU--------AGGUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 25628 | 0.68 | 0.20646 |
Target: 5'- aUGGGUaGCCccuuGGGUGCGGAugaucaugucgaccuUCgCGGCGCa -3' miRNA: 3'- aACUCG-CGG----UCCGCGCCU---------------AG-GUCGCG- -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 17418 | 0.68 | 0.204257 |
Target: 5'- cUGGGCGUCAGGCGuuGAcgcggCUGGCGa -3' miRNA: 3'- aACUCGCGGUCCGCgcCUa----GGUCGCg -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 9617 | 0.68 | 0.193547 |
Target: 5'- ---uGCGCCAGGCGCuGGccAUCC-GCGa -3' miRNA: 3'- aacuCGCGGUCCGCG-CC--UAGGuCGCg -5' |
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11914 | 3' | -61.1 | NC_003278.1 | + | 26480 | 0.68 | 0.188378 |
Target: 5'- -cGAGCguucGCCAGGCGCuGGUgcUCGGCGg -3' miRNA: 3'- aaCUCG----CGGUCCGCGcCUA--GGUCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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