Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1192 | 3' | -54.9 | NC_001132.2 | + | 3418 | 0.66 | 0.956574 |
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Target: 5'- gGAACgaauUCgGCGCGGACGACagCGUGa -3' miRNA: 3'- aUUUGgc--AGgCGCGCUUGCUGa-GCGC- -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 9306 | 0.66 | 0.956195 |
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Target: 5'- cGAACUGUCCGUccccaagGCGAGCGuuACg-GCGa -3' miRNA: 3'- aUUUGGCAGGCG-------CGCUUGC--UGagCGC- -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 49709 | 0.66 | 0.952686 |
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Target: 5'- -uAAUCGUCUGC-UGggUGGCUCGUu -3' miRNA: 3'- auUUGGCAGGCGcGCuuGCUGAGCGc -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 11669 | 0.67 | 0.934833 |
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Target: 5'- cGAGUCGUUCGCGCGGACGGuuuauaCGUGg -3' miRNA: 3'- aUUUGGCAGGCGCGCUUGCUga----GCGC- -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 151482 | 0.67 | 0.913208 |
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Target: 5'- --uGCCuUCCGCGaCGAuuuCGACgUCGCa -3' miRNA: 3'- auuUGGcAGGCGC-GCUu--GCUG-AGCGc -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 87114 | 0.7 | 0.800685 |
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Target: 5'- cAAACUGUCUGUGCGggUuGugUuCGCGu -3' miRNA: 3'- aUUUGGCAGGCGCGCuuG-CugA-GCGC- -5' |
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| 1192 | 3' | -54.9 | NC_001132.2 | + | 150290 | 1.07 | 0.005794 |
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Target: 5'- aUAAACCGUCCGCGCGAACGACUCGCGu -3' miRNA: 3'- -AUUUGGCAGGCGCGCUUGCUGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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