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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1194 | 5' | -44.1 | NC_001132.2 | + | 129932 | 0.71 | 0.999382 |
|
Target: 5'- aGGUaAUUAGAccgagcGUCCUCCGUAAc---- -3' miRNA: 3'- aCCA-UAAUCU------CAGGAGGCAUUauauc -5' |
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| 1194 | 5' | -44.1 | NC_001132.2 | + | 89917 | 0.66 | 1 |
|
Target: 5'- aUGGUGUUGGAGUCacaaCC-UAAUAa-- -3' miRNA: 3'- -ACCAUAAUCUCAGga--GGcAUUAUauc -5' |
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| 1194 | 5' | -44.1 | NC_001132.2 | + | 4768 | 0.69 | 0.999918 |
|
Target: 5'- gGGUAggaaccaUGGAGUCCUUCGUGc----- -3' miRNA: 3'- aCCAUa------AUCUCAGGAGGCAUuauauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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