Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11971 | 3' | -55.8 | NC_003278.1 | + | 32147 | 0.72 | 0.24873 |
Target: 5'- aCUGCGGCGagcugggcggucUGGCCcagucGCGGAAGCGCu -3' miRNA: 3'- aGAUGCCGC------------GUCGGu----UGUCUUCGCGc -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 21413 | 0.73 | 0.2114 |
Target: 5'- --cGCGGCGCcGCCGACgcgcuGGAcGGCGCGc -3' miRNA: 3'- agaUGCCGCGuCGGUUG-----UCU-UCGCGC- -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 6687 | 0.74 | 0.169212 |
Target: 5'- cUCUuCGGCgaugacGCAGCCGGCGGuacGCGCGa -3' miRNA: 3'- -AGAuGCCG------CGUCGGUUGUCuu-CGCGC- -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 31681 | 0.75 | 0.155435 |
Target: 5'- cCUGCGGCGCcGCCGcccaggccgcaGCAGAAGUGg- -3' miRNA: 3'- aGAUGCCGCGuCGGU-----------UGUCUUCGCgc -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 33263 | 0.75 | 0.155435 |
Target: 5'- cCU-CGGcCGCAGCCGGCGGAAcCGCa -3' miRNA: 3'- aGAuGCC-GCGUCGGUUGUCUUcGCGc -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 35529 | 0.75 | 0.13825 |
Target: 5'- uUCUGCGGacccCAGCCAGCAGGcuuggcgGGCGCu -3' miRNA: 3'- -AGAUGCCgc--GUCGGUUGUCU-------UCGCGc -5' |
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11971 | 3' | -55.8 | NC_003278.1 | + | 10546 | 1.09 | 0.000456 |
Target: 5'- aUCUACGGCGCAGCCAACAGAAGCGCGu -3' miRNA: 3'- -AGAUGCCGCGUCGGUUGUCUUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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