Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12095 | 3' | -57.7 | NC_003309.1 | + | 52724 | 0.66 | 0.561329 |
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Target: 5'- aGuuGGCGggCCAGcCCGGCuaccGGCUCc -3' miRNA: 3'- gUggCUGUuaGGUCaGGCCG----CCGAGc -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 31560 | 0.67 | 0.550749 |
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Target: 5'- cCACCGGCAAUUUucGUacgcaCGGCGGCa-- -3' miRNA: 3'- -GUGGCUGUUAGGu-CAg----GCCGCCGagc -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 9574 | 0.67 | 0.509116 |
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Target: 5'- aCGCCGAacg-CCGcUgCGGCGGCUUGu -3' miRNA: 3'- -GUGGCUguuaGGUcAgGCCGCCGAGC- -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 47397 | 0.67 | 0.509116 |
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Target: 5'- -gUCGACGGgcaaCAGUCgGGCaGCUCGa -3' miRNA: 3'- guGGCUGUUag--GUCAGgCCGcCGAGC- -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 18692 | 0.68 | 0.48779 |
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Target: 5'- gGCCGcCGAUCCGGcggCCGGCcuucuuaGGgUCGg -3' miRNA: 3'- gUGGCuGUUAGGUCa--GGCCG-------CCgAGC- -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 32061 | 0.68 | 0.47878 |
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Target: 5'- aCGCCGaggcgcGCAAUgCAcgcuucgCUGGCGGCUCGg -3' miRNA: 3'- -GUGGC------UGUUAgGUca-----GGCCGCCGAGC- -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 11494 | 0.74 | 0.197473 |
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Target: 5'- uCGCCGuguCGAUCgcg-CCGGCGGCUCGa -3' miRNA: 3'- -GUGGCu--GUUAGgucaGGCCGCCGAGC- -5' |
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| 12095 | 3' | -57.7 | NC_003309.1 | + | 256 | 1.08 | 0.000678 |
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Target: 5'- uCACCGACAAUCCAGUCCGGCGGCUCGa -3' miRNA: 3'- -GUGGCUGUUAGGUCAGGCCGCCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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