Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12102 | 5' | -60.3 | NC_003309.1 | + | 12645 | 0.66 | 0.492234 |
Target: 5'- gCCGCU-GCUCGAGauaGCCGcGcUCGCg -3' miRNA: 3'- aGGCGGuCGAGCUUgcgCGGC-C-AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 22477 | 0.66 | 0.434644 |
Target: 5'- aCCGCCgacgcggcGGCaUCGuGCGCGgCGaUCGCa -3' miRNA: 3'- aGGCGG--------UCG-AGCuUGCGCgGCcAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10755 | 0.66 | 0.443975 |
Target: 5'- gCCGCCGcCUCaa--GCGCCGuccGUCGCu -3' miRNA: 3'- aGGCGGUcGAGcuugCGCGGC---CAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 13181 | 0.66 | 0.443975 |
Target: 5'- cCCGCCAacaagcccacccGCggCGAaucGCGCgaaGCCGGaUCGCc -3' miRNA: 3'- aGGCGGU------------CGa-GCU---UGCG---CGGCC-AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10252 | 0.66 | 0.443975 |
Target: 5'- aUCCGCCcAGCcgCGAGCGacaCGCCGa--GCu -3' miRNA: 3'- -AGGCGG-UCGa-GCUUGC---GCGGCcagCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 22854 | 0.66 | 0.443975 |
Target: 5'- -gCGaCCGGCaUGAguguucccgcACGCacGCCGGUCGCc -3' miRNA: 3'- agGC-GGUCGaGCU----------UGCG--CGGCCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 242 | 0.66 | 0.462009 |
Target: 5'- gUCCGgCGGCUCGAuguuCGUGaccaagccgaaauCCGGcUCGUc -3' miRNA: 3'- -AGGCgGUCGAGCUu---GCGC-------------GGCC-AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 53270 | 0.66 | 0.462969 |
Target: 5'- aCCGCaaccGCUC--GCGCGCCGucuUCGCc -3' miRNA: 3'- aGGCGgu--CGAGcuUGCGCGGCc--AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 47961 | 0.66 | 0.462969 |
Target: 5'- -gCGCCAGCUCGcgcucggcgaAGCuGCGaCCGauaguucggucGUCGCg -3' miRNA: 3'- agGCGGUCGAGC----------UUG-CGC-GGC-----------CAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 21994 | 0.66 | 0.462969 |
Target: 5'- cUCCGCguGCUCGAuga-GCC-GUUGCu -3' miRNA: 3'- -AGGCGguCGAGCUugcgCGGcCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 49487 | 0.66 | 0.462969 |
Target: 5'- cUCCGCU--CUCGAugaAUGCGCCGaGcacgCGCg -3' miRNA: 3'- -AGGCGGucGAGCU---UGCGCGGC-Ca---GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 39617 | 0.66 | 0.482381 |
Target: 5'- gCUGCCAaGCaugCGAGCGUGCUGcGcuUCGUg -3' miRNA: 3'- aGGCGGU-CGa--GCUUGCGCGGC-C--AGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 43294 | 0.66 | 0.482381 |
Target: 5'- cCCaUCAGUUCG-ACGaugGCCGcGUCGCa -3' miRNA: 3'- aGGcGGUCGAGCuUGCg--CGGC-CAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 40473 | 0.66 | 0.492234 |
Target: 5'- --aGCCAGa--GGACGCcggaGuuGGUCGCg -3' miRNA: 3'- aggCGGUCgagCUUGCG----CggCCAGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 10360 | 0.67 | 0.410925 |
Target: 5'- gCCGCCucguguuGCUugaucgucuccucgaCGAGCGCucGCCGGgCGCc -3' miRNA: 3'- aGGCGGu------CGA---------------GCUUGCG--CGGCCaGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 7239 | 0.67 | 0.415424 |
Target: 5'- aCCGaCAGgUCGAgauacccgcguuuGCGCGCCGGcaguacCGCc -3' miRNA: 3'- aGGCgGUCgAGCU-------------UGCGCGGCCa-----GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 27752 | 0.67 | 0.416327 |
Target: 5'- gUCGCCAGa-CG-ACGCGCCGcccggCGCa -3' miRNA: 3'- aGGCGGUCgaGCuUGCGCGGCca---GCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 513 | 0.67 | 0.416327 |
Target: 5'- cUCGCCGGgUCGAGCcccagcauuGCGCCGaaGcCGCc -3' miRNA: 3'- aGGCGGUCgAGCUUG---------CGCGGC--CaGCG- -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 33335 | 0.67 | 0.425427 |
Target: 5'- gUCCGCCuaccaauuGCUUcgGGGCGuCGCCGGUg-- -3' miRNA: 3'- -AGGCGGu-------CGAG--CUUGC-GCGGCCAgcg -5' |
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12102 | 5' | -60.3 | NC_003309.1 | + | 11792 | 0.67 | 0.407348 |
Target: 5'- aUCGCCuuUUCGAgcgacuuguACGCGUCGG-CGCg -3' miRNA: 3'- aGGCGGucGAGCU---------UGCGCGGCCaGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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