Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12104 | 3' | -57.5 | NC_003309.1 | + | 22476 | 0.66 | 0.549647 |
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Target: 5'- cCGCCgacgcggcggCaUCGUGCGCGgCGAUCg -3' miRNA: 3'- uGCGGaaca------GaAGCGCGCGCgGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 17757 | 0.66 | 0.543266 |
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Target: 5'- -aGCCgu-UUUUCGCGCGCGgcagcUCGAUCc -3' miRNA: 3'- ugCGGaacAGAAGCGCGCGC-----GGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 37296 | 0.67 | 0.532687 |
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Target: 5'- gACGCCcUGaaUC-UCGCcgGCGCGUCGAUg -3' miRNA: 3'- -UGCGGaAC--AGaAGCG--CGCGCGGCUAg -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 10611 | 0.67 | 0.522182 |
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Target: 5'- cUGCCggcaacgUUCGCGCGUGCCG-UCg -3' miRNA: 3'- uGCGGaacag--AAGCGCGCGCGGCuAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 19303 | 0.67 | 0.522182 |
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Target: 5'- cACGCCgagcgcGUUUUCG-GCGCGCaCGcgCg -3' miRNA: 3'- -UGCGGaa----CAGAAGCgCGCGCG-GCuaG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 44493 | 0.67 | 0.511758 |
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Target: 5'- uGCGCuaCUUGUCcggUCGUGcCGCuGCCGAa- -3' miRNA: 3'- -UGCG--GAACAGa--AGCGC-GCG-CGGCUag -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 45971 | 0.67 | 0.511758 |
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Target: 5'- aACGaCUUGUCgcugUCGCGCaugacGCGCUucggGAUCg -3' miRNA: 3'- -UGCgGAACAGa---AGCGCG-----CGCGG----CUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 22365 | 0.67 | 0.501422 |
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Target: 5'- -gGCCUgcucGUCggcaagcCGaUGUGCGCCGAUCa -3' miRNA: 3'- ugCGGAa---CAGaa-----GC-GCGCGCGGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 43667 | 0.67 | 0.501422 |
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Target: 5'- cACGCCgaccagcgUGUUgaugaacUCGCGCaGCGUCGGUUc -3' miRNA: 3'- -UGCGGa-------ACAGa------AGCGCG-CGCGGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 5574 | 1.09 | 0.000516 |
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Target: 5'- aACGCCUUGUCUUCGCGCGCGCCGAUCg -3' miRNA: 3'- -UGCGGAACAGAAGCGCGCGCGGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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