Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12104 | 3' | -57.5 | NC_003309.1 | + | 19303 | 0.67 | 0.522182 |
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Target: 5'- cACGCCgagcgcGUUUUCG-GCGCGCaCGcgCg -3' miRNA: 3'- -UGCGGaa----CAGAAGCgCGCGCG-GCuaG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 10611 | 0.67 | 0.522182 |
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Target: 5'- cUGCCggcaacgUUCGCGCGUGCCG-UCg -3' miRNA: 3'- uGCGGaacag--AAGCGCGCGCGGCuAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 37296 | 0.67 | 0.532687 |
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Target: 5'- gACGCCcUGaaUC-UCGCcgGCGCGUCGAUg -3' miRNA: 3'- -UGCGGaAC--AGaAGCG--CGCGCGGCUAg -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 17757 | 0.66 | 0.543266 |
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Target: 5'- -aGCCgu-UUUUCGCGCGCGgcagcUCGAUCc -3' miRNA: 3'- ugCGGaacAGAAGCGCGCGC-----GGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 22476 | 0.66 | 0.549647 |
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Target: 5'- cCGCCgacgcggcggCaUCGUGCGCGgCGAUCg -3' miRNA: 3'- uGCGGaaca------GaAGCGCGCGCgGCUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 47613 | 0.66 | 0.564622 |
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Target: 5'- aACGuCCUgcccgGUCgUCGC-CGCGCCG-UCc -3' miRNA: 3'- -UGC-GGAa----CAGaAGCGcGCGCGGCuAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 7425 | 0.66 | 0.573228 |
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Target: 5'- uGCGCCgcaacuucccgGUCUUCuucgGCGCGCGCUu--- -3' miRNA: 3'- -UGCGGaa---------CAGAAG----CGCGCGCGGcuag -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 22439 | 0.66 | 0.575384 |
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Target: 5'- cUGCCggcGUCgagCGCgGCGCGCgaGAUCu -3' miRNA: 3'- uGCGGaa-CAGaa-GCG-CGCGCGg-CUAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 47714 | 0.66 | 0.581864 |
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Target: 5'- cGCGCCUUGaaacgcaugaacaCUUCGCccuCGCGCUGcUCg -3' miRNA: 3'- -UGCGGAACa------------GAAGCGc--GCGCGGCuAG- -5' |
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| 12104 | 3' | -57.5 | NC_003309.1 | + | 31713 | 0.66 | 0.586192 |
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Target: 5'- -gGCCgcaUGUCUggcUCG-GCGCGaCGAUCg -3' miRNA: 3'- ugCGGa--ACAGA---AGCgCGCGCgGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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