Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12104 | 5' | -55 | NC_003309.1 | + | 26844 | 0.66 | 0.72126 |
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Target: 5'- cGGGGUCGCCGACaucgagccccggCAUaAUUUGcgCGAu -3' miRNA: 3'- -CUCCAGCGGCUG------------GUGcUAGACuaGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 48216 | 0.66 | 0.710591 |
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Target: 5'- cGAGcGcCGCUGACgCGCGAUCaGcUCGAa -3' miRNA: 3'- -CUC-CaGCGGCUG-GUGCUAGaCuAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 12843 | 0.67 | 0.689033 |
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Target: 5'- cGAGGUCGgCGAUCAUG--CUG-UCGAu -3' miRNA: 3'- -CUCCAGCgGCUGGUGCuaGACuAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 44863 | 0.67 | 0.667257 |
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Target: 5'- cAGGuUCGCCGGCgGCGAUCcGcgCa- -3' miRNA: 3'- cUCC-AGCGGCUGgUGCUAGaCuaGcu -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 11154 | 0.69 | 0.536587 |
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Target: 5'- --aGUUGCCGAUauuugucgAUGAUCUGAUCGAg -3' miRNA: 3'- cucCAGCGGCUGg-------UGCUAGACUAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 19768 | 0.71 | 0.434855 |
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Target: 5'- --cGUCGCCGGCCAUcggcgggguGAUCaGGUCGAu -3' miRNA: 3'- cucCAGCGGCUGGUG---------CUAGaCUAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 8708 | 0.72 | 0.374147 |
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Target: 5'- -cGGUCGCCGACCgucggcucgcgcacuGCGAUCUuuUCGc -3' miRNA: 3'- cuCCAGCGGCUGG---------------UGCUAGAcuAGCu -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 49095 | 0.74 | 0.321014 |
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Target: 5'- -cGGcgUGCCGACC-CGGUuCUGAUCGAu -3' miRNA: 3'- cuCCa-GCGGCUGGuGCUA-GACUAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 48016 | 0.75 | 0.243315 |
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Target: 5'- cGAGGUCGCCGGauGCGAUUUcGUCGAa -3' miRNA: 3'- -CUCCAGCGGCUggUGCUAGAcUAGCU- -5' |
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| 12104 | 5' | -55 | NC_003309.1 | + | 5540 | 1.08 | 0.001294 |
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Target: 5'- uGAGGUCGCCGACCACGAUCUGAUCGAc -3' miRNA: 3'- -CUCCAGCGGCUGGUGCUAGACUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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