Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12108 | 3' | -53.5 | NC_003309.1 | + | 474 | 0.66 | 0.78089 |
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Target: 5'- -----uUUGCGCCGCcGCUUCCuucacaaCGGUc -3' miRNA: 3'- ugcuauAACGCGGCGuUGAAGG-------GCCA- -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 164 | 0.67 | 0.750826 |
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Target: 5'- cGCGAUuuuccGCGCCGuCGGCUUggguuuucugcCCCGGc -3' miRNA: 3'- -UGCUAuaa--CGCGGC-GUUGAA-----------GGGCCa -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 28839 | 0.67 | 0.750826 |
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Target: 5'- cACGGUGcugGCGCUGCAACcaugucgCCCGcGg -3' miRNA: 3'- -UGCUAUaa-CGCGGCGUUGaa-----GGGC-Ca -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 26474 | 0.67 | 0.718618 |
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Target: 5'- cGCGAacgGcUGCGCCGC--CUUCCCGu- -3' miRNA: 3'- -UGCUa--UaACGCGGCGuuGAAGGGCca -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 44561 | 0.68 | 0.696679 |
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Target: 5'- gACGAUc--GCGCUGCAGCggcucauagUCCgGGUu -3' miRNA: 3'- -UGCUAuaaCGCGGCGUUGa--------AGGgCCA- -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 23985 | 0.68 | 0.640899 |
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Target: 5'- cACGAgcggaacGCGCCGCGACga-UCGGUg -3' miRNA: 3'- -UGCUauaa---CGCGGCGUUGaagGGCCA- -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 7773 | 0.69 | 0.629674 |
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Target: 5'- gACGAaaUAcgGCGCCgGCGcCUUCgCCGGUg -3' miRNA: 3'- -UGCU--AUaaCGCGG-CGUuGAAG-GGCCA- -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 13800 | 0.72 | 0.417009 |
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Target: 5'- cGCGGuUGUUGuCGCCGCcGCUUCcgCCGGg -3' miRNA: 3'- -UGCU-AUAAC-GCGGCGuUGAAG--GGCCa -5' |
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| 12108 | 3' | -53.5 | NC_003309.1 | + | 7433 | 1.07 | 0.001964 |
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Target: 5'- gACGAUAUUGCGCCGCAACUUCCCGGUc -3' miRNA: 3'- -UGCUAUAACGCGGCGUUGAAGGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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