Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12108 | 5' | -56 | NC_003309.1 | + | 5559 | 0.66 | 0.64419 |
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Target: 5'- -cGCGCGCCga-UCGCGGcggugagGUCGCCg- -3' miRNA: 3'- aaUGCGCGGagcAGUGCC-------UAGUGGaa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 45948 | 0.66 | 0.634147 |
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Target: 5'- -gACGCGCUUCG----GGAUCGCCg- -3' miRNA: 3'- aaUGCGCGGAGCagugCCUAGUGGaa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 9529 | 0.68 | 0.471595 |
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Target: 5'- cUGCGCGCCgCGUCgaGCGcGcgCACCg- -3' miRNA: 3'- aAUGCGCGGaGCAG--UGC-CuaGUGGaa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 19021 | 0.69 | 0.451373 |
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Target: 5'- cUGCGCGCCgUCGUC---GAUCGCCg- -3' miRNA: 3'- aAUGCGCGG-AGCAGugcCUAGUGGaa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 39599 | 0.69 | 0.440455 |
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Target: 5'- gUGCuGCGCUUCGUgACGGAUucaagaucgaaucCGCCUg -3' miRNA: 3'- aAUG-CGCGGAGCAgUGCCUA-------------GUGGAa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 6549 | 0.71 | 0.349482 |
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Target: 5'- -aGCGCGCCUUGcguaUCGCGGcgCcaGCCUUg -3' miRNA: 3'- aaUGCGCGGAGC----AGUGCCuaG--UGGAA- -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 47858 | 0.77 | 0.146143 |
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Target: 5'- -aGCGCGCUUCGUCACG--UCGCCUc -3' miRNA: 3'- aaUGCGCGGAGCAGUGCcuAGUGGAa -5' |
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| 12108 | 5' | -56 | NC_003309.1 | + | 7391 | 1.02 | 0.00206 |
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Target: 5'- cUUACGCGCCUCGUCACGGAUCACCUUc -3' miRNA: 3'- -AAUGCGCGGAGCAGUGCCUAGUGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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