Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1211 | 3' | -56.6 | NC_001132.2 | + | 126191 | 0.66 | 0.913085 |
Target: 5'- cUUCGGGaGUugGgGUAgGGGCGGuCGUc -3' miRNA: 3'- -AGGCCUgUAugCgCAUgCCCGCU-GCA- -5' |
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1211 | 3' | -56.6 | NC_001132.2 | + | 149579 | 0.66 | 0.911339 |
Target: 5'- aUUCGGAUAuauaccuaaccauUACGUGUcuggaaccggaGGGCGACGUg -3' miRNA: 3'- -AGGCCUGU-------------AUGCGCAug---------CCCGCUGCA- -5' |
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1211 | 3' | -56.6 | NC_001132.2 | + | 30094 | 0.67 | 0.870065 |
Target: 5'- aCCGGACG-ACGuCGUACcuagucuguugcaauGGGCGAgCGa -3' miRNA: 3'- aGGCCUGUaUGC-GCAUG---------------CCCGCU-GCa -5' |
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1211 | 3' | -56.6 | NC_001132.2 | + | 27488 | 0.67 | 0.867158 |
Target: 5'- -aCGaGACG-ACGCGUACGGGUauACGUa -3' miRNA: 3'- agGC-CUGUaUGCGCAUGCCCGc-UGCA- -5' |
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1211 | 3' | -56.6 | NC_001132.2 | + | 2400 | 1.08 | 0.003719 |
Target: 5'- cUCCGGACAUACGCGUACGGGCGACGUg -3' miRNA: 3'- -AGGCCUGUAUGCGCAUGCCCGCUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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