Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12112 | 3' | -54.2 | NC_003309.1 | + | 24271 | 0.69 | 0.591564 |
Target: 5'- gCCUGCUuCUcGGGUgUGUcgauguaGCCCGUCa -3' miRNA: 3'- -GGACGGuGA-CCUAgACAaa-----CGGGCAG- -5' |
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12112 | 3' | -54.2 | NC_003309.1 | + | 19038 | 0.7 | 0.525974 |
Target: 5'- aUCgGCCguuGCUGGAUCUGc--GCgCCGUCg -3' miRNA: 3'- -GGaCGG---UGACCUAGACaaaCG-GGCAG- -5' |
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12112 | 3' | -54.2 | NC_003309.1 | + | 23940 | 0.74 | 0.310436 |
Target: 5'- aCUUGaUCGCUGGAcuggacgCUGUcgGCCCGUCg -3' miRNA: 3'- -GGAC-GGUGACCUa------GACAaaCGGGCAG- -5' |
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12112 | 3' | -54.2 | NC_003309.1 | + | 8223 | 1.12 | 0.000678 |
Target: 5'- cCCUGCCACUGGAUCUGUUUGCCCGUCg -3' miRNA: 3'- -GGACGGUGACCUAGACAAACGGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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