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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12119 | 5' | -51.8 | NC_003309.1 | + | 19038 | 0.66 | 0.897692 |
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Target: 5'- aUCGGcCGUUGCuGGAucuGCGcGCcgUCGUCGa -3' miRNA: 3'- gAGCC-GUAACG-CCU---UGCuUG--AGCAGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 4411 | 0.66 | 0.89041 |
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Target: 5'- -gCGGCucaUGCGGggUGAGaUCGUUc -3' miRNA: 3'- gaGCCGua-ACGCCuuGCUUgAGCAGu -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 22398 | 0.66 | 0.88892 |
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Target: 5'- uUCGGCAUgccgcUGCGcgucGAACGcccgcgcggccuGCUCGUCGg -3' miRNA: 3'- gAGCCGUA-----ACGC----CUUGCu-----------UGAGCAGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 43499 | 0.66 | 0.875025 |
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Target: 5'- -gCGGCAgucuccGCGGuGCGAACg-GUCAa -3' miRNA: 3'- gaGCCGUaa----CGCCuUGCUUGagCAGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 33800 | 0.66 | 0.858589 |
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Target: 5'- gUUGGCug-GCGGGuC-AGCUCGUCGa -3' miRNA: 3'- gAGCCGuaaCGCCUuGcUUGAGCAGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 13081 | 0.68 | 0.803625 |
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Target: 5'- -gCGGC---GCGGAccACGAACUCGcCGu -3' miRNA: 3'- gaGCCGuaaCGCCU--UGCUUGAGCaGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 23021 | 0.68 | 0.773461 |
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Target: 5'- uUCGGaaagcGCGGGAUGAugUgGUCAg -3' miRNA: 3'- gAGCCguaa-CGCCUUGCUugAgCAGU- -5' |
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| 12119 | 5' | -51.8 | NC_003309.1 | + | 12768 | 1.1 | 0.002113 |
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Target: 5'- cCUCGGCAUUGCGGAACGAACUCGUCAg -3' miRNA: 3'- -GAGCCGUAACGCCUUGCUUGAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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