Results 21 - 38 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12125 | 3' | -53 | NC_003309.1 | + | 47937 | 0.68 | 0.720944 |
Target: 5'- uGCGAccGAUAguUCGgucGUCGCGCACGcgUGCc -3' miRNA: 3'- -CGCU--CUAU--GGU---CAGCGCGUGCuaGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 33063 | 0.68 | 0.720944 |
Target: 5'- cGCGuucGAUuauuGCgAcUCGCGCACGAUCGa -3' miRNA: 3'- -CGCu--CUA----UGgUcAGCGCGUGCUAGCg -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 19296 | 0.68 | 0.742192 |
Target: 5'- aGCGcGuuuUCGG-CGCGCACGcgCGCg -3' miRNA: 3'- -CGCuCuauGGUCaGCGCGUGCuaGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 6044 | 0.68 | 0.742192 |
Target: 5'- cGCGAaucagcguCUGGUCGCGCugGAaUGCa -3' miRNA: 3'- -CGCUcuau----GGUCAGCGCGugCUaGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 43536 | 0.68 | 0.752654 |
Target: 5'- cGCGcacgccgucGGcgGCCA-UCGCGCGCaucaGGUCGCg -3' miRNA: 3'- -CGC---------UCuaUGGUcAGCGCGUG----CUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 37334 | 0.68 | 0.752654 |
Target: 5'- aUGAGAUcACgGGcgucuaccUGCGCGCGAUCGUg -3' miRNA: 3'- cGCUCUA-UGgUCa-------GCGCGUGCUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 4179 | 0.7 | 0.632186 |
Target: 5'- aGCGcGGAUgucguccGCCAGcucgaaCGCGC-CGGUCGCg -3' miRNA: 3'- -CGC-UCUA-------UGGUCa-----GCGCGuGCUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 12637 | 0.7 | 0.611157 |
Target: 5'- uCGAGAUaGCCGcGcUCGCGCGCGAccUGCu -3' miRNA: 3'- cGCUCUA-UGGU-C-AGCGCGUGCUa-GCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 40117 | 0.71 | 0.556295 |
Target: 5'- aGCGAGAacAUCGGUCGCGCccaugcaauGCGGcaCGCg -3' miRNA: 3'- -CGCUCUa-UGGUCAGCGCG---------UGCUa-GCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 18164 | 0.71 | 0.567178 |
Target: 5'- ---cGAUACCAGcUCGUcacagugacGCGCGAUUGCg -3' miRNA: 3'- cgcuCUAUGGUC-AGCG---------CGUGCUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 44782 | 0.71 | 0.567178 |
Target: 5'- cCGGGAUACCGa--GCGCGCGAg-GCa -3' miRNA: 3'- cGCUCUAUGGUcagCGCGUGCUagCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 48725 | 0.71 | 0.578113 |
Target: 5'- aGCGAaucaucaggucGAcUGCCAaugccgCGUGCGCGAUCGCu -3' miRNA: 3'- -CGCU-----------CU-AUGGUca----GCGCGUGCUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 28449 | 0.73 | 0.472018 |
Target: 5'- cCGAGGUugUcGUCGCGCACGuucaugUGCu -3' miRNA: 3'- cGCUCUAugGuCAGCGCGUGCua----GCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 43429 | 0.73 | 0.432344 |
Target: 5'- cGCGAGAUugaacagcACCuGUgCGUGCugGAagaUCGCg -3' miRNA: 3'- -CGCUCUA--------UGGuCA-GCGCGugCU---AGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 19956 | 0.74 | 0.422733 |
Target: 5'- cGCGcGAU-CCGcuucGaCGCGCGCGAUCGCu -3' miRNA: 3'- -CGCuCUAuGGU----CaGCGCGUGCUAGCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 27822 | 0.78 | 0.24039 |
Target: 5'- uGCGAGA-ACCGGUCGCGCccuuuucggACGAguggCGUg -3' miRNA: 3'- -CGCUCUaUGGUCAGCGCG---------UGCUa---GCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 9709 | 0.78 | 0.228089 |
Target: 5'- aGCGGu-UGCCGuguGUCGCGCGCGAUgCGCa -3' miRNA: 3'- -CGCUcuAUGGU---CAGCGCGUGCUA-GCG- -5' |
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12125 | 3' | -53 | NC_003309.1 | + | 16287 | 1.15 | 0.000731 |
Target: 5'- aGCGAGAUACCAGUCGCGCACGAUCGCg -3' miRNA: 3'- -CGCUCUAUGGUCAGCGCGUGCUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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