Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12130 | 5' | -54.9 | NC_003309.1 | + | 39016 | 0.75 | 0.267107 |
Target: 5'- -aUCGAGC-UCAUGCCGuCcGGCGCCa -3' miRNA: 3'- gcAGCUUGcAGUACGGCuGcUCGCGG- -5' |
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12130 | 5' | -54.9 | NC_003309.1 | + | 45263 | 0.76 | 0.253909 |
Target: 5'- gGUCGAAUG-CcgGCCGccGCGuGCGCCu -3' miRNA: 3'- gCAGCUUGCaGuaCGGC--UGCuCGCGG- -5' |
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12130 | 5' | -54.9 | NC_003309.1 | + | 17512 | 0.76 | 0.235124 |
Target: 5'- gCGUCGGACGUCAgcgGCaCGcCGA-CGCCg -3' miRNA: 3'- -GCAGCUUGCAGUa--CG-GCuGCUcGCGG- -5' |
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12130 | 5' | -54.9 | NC_003309.1 | + | 22382 | 0.76 | 0.233311 |
Target: 5'- gCGUCGAACGcccgcgcggccugcUCGUcggcaaGCCGAUGuGCGCCg -3' miRNA: 3'- -GCAGCUUGC--------------AGUA------CGGCUGCuCGCGG- -5' |
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12130 | 5' | -54.9 | NC_003309.1 | + | 18435 | 0.82 | 0.101623 |
Target: 5'- uCGUCGGAuCGUUGUaGCUGACGAGCGCg -3' miRNA: 3'- -GCAGCUU-GCAGUA-CGGCUGCUCGCGg -5' |
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12130 | 5' | -54.9 | NC_003309.1 | + | 17899 | 1.13 | 0.000723 |
Target: 5'- gCGUCGAACGUCAUGCCGACGAGCGCCg -3' miRNA: 3'- -GCAGCUUGCAGUACGGCUGCUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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