Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12133 | 5' | -53 | NC_003309.1 | + | 22441 | 0.66 | 0.803241 |
Target: 5'- cGCUGCCG---GCGUCGAgCGcGGCGcGCg -3' miRNA: 3'- -UGAUGGCucaUGCAGCU-GU-CCGCaUG- -5' |
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12133 | 5' | -53 | NC_003309.1 | + | 5744 | 0.67 | 0.783462 |
Target: 5'- --aGCCGGGcUGCGUCaGAUuGGCGUcaGCg -3' miRNA: 3'- ugaUGGCUC-AUGCAG-CUGuCCGCA--UG- -5' |
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12133 | 5' | -53 | NC_003309.1 | + | 32790 | 0.67 | 0.763037 |
Target: 5'- gGCUGCUGA-UGCGUUGgACAGGCucggACa -3' miRNA: 3'- -UGAUGGCUcAUGCAGC-UGUCCGca--UG- -5' |
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12133 | 5' | -53 | NC_003309.1 | + | 16538 | 0.69 | 0.654519 |
Target: 5'- --cACCGcGcGCGUCGAUAGGCG-ACc -3' miRNA: 3'- ugaUGGCuCaUGCAGCUGUCCGCaUG- -5' |
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12133 | 5' | -53 | NC_003309.1 | + | 52700 | 0.71 | 0.56553 |
Target: 5'- gGCU-CCGAGUugG-CGAUuGGCGUGu -3' miRNA: 3'- -UGAuGGCUCAugCaGCUGuCCGCAUg -5' |
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12133 | 5' | -53 | NC_003309.1 | + | 19105 | 1.1 | 0.001375 |
Target: 5'- gACUACCGAGUACGUCGACAGGCGUACg -3' miRNA: 3'- -UGAUGGCUCAUGCAGCUGUCCGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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