Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12134 | 3' | -58.5 | NC_003309.1 | + | 26951 | 0.69 | 0.432363 |
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Target: 5'- --cGUCAGCG-CGCGaacaccGCCGACGGUGu -3' miRNA: 3'- cgcCAGUCGCuGUGC------UGGCUGCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 28197 | 0.66 | 0.570937 |
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Target: 5'- --cGUCAGgGGCGCGAUaguuggugcacaCGACGGCa- -3' miRNA: 3'- cgcCAGUCgCUGUGCUG------------GCUGCCGcc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 31476 | 0.67 | 0.53993 |
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Target: 5'- cCGG-CGGCGAaaGCGGCC-ACGGCGc -3' miRNA: 3'- cGCCaGUCGCUg-UGCUGGcUGCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 31537 | 0.67 | 0.519576 |
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Target: 5'- gGCGGcaAGCGccagcucgaacuACGCcggccaGCCGACGGCGGc -3' miRNA: 3'- -CGCCagUCGC------------UGUGc-----UGGCUGCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 37947 | 0.66 | 0.570937 |
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Target: 5'- cGCGGgauuuccucUCGGauaaGCGCGGCCuuuCGGCGGg -3' miRNA: 3'- -CGCC---------AGUCgc--UGUGCUGGcu-GCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 40187 | 0.69 | 0.441641 |
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Target: 5'- aGCGGaccucgaagUCAGCGcaaACGCGAgccgugagaCGugGGCGGu -3' miRNA: 3'- -CGCC---------AGUCGC---UGUGCUg--------GCugCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 43563 | 0.66 | 0.575107 |
|
Target: 5'- aCGG-CGGCGacgugacgaucgucuGCACGcgcacGCCGuCGGCGGc -3' miRNA: 3'- cGCCaGUCGC---------------UGUGC-----UGGCuGCCGCC- -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 46112 | 0.71 | 0.308057 |
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Target: 5'- aCGGUCGGCaccucGACAcCGAacgCGACGGCGa -3' miRNA: 3'- cGCCAGUCG-----CUGU-GCUg--GCUGCCGCc -5' |
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| 12134 | 3' | -58.5 | NC_003309.1 | + | 47491 | 0.69 | 0.405228 |
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Target: 5'- gGCGGcgCAGCGACACcuucGCCGcCgGGCGu -3' miRNA: 3'- -CGCCa-GUCGCUGUGc---UGGCuG-CCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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