Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12137 | 5' | -52.7 | NC_003309.1 | + | 2279 | 0.66 | 0.832664 |
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Target: 5'- aUCGACuc-GGaauCGACGCGAUCGGCg -3' miRNA: 3'- -AGUUGcauUCgcuGCUGCGCUGGCUG- -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 37590 | 0.66 | 0.841592 |
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Target: 5'- gCAACGcuacGGCGACGAcCGCGGCg--- -3' miRNA: 3'- aGUUGCau--UCGCUGCU-GCGCUGgcug -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 18433 | 0.66 | 0.850296 |
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Target: 5'- gUCGgauCGUuguAGCuGACGAgCGCGACCGu- -3' miRNA: 3'- -AGUu--GCAu--UCG-CUGCU-GCGCUGGCug -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 52479 | 0.66 | 0.850296 |
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Target: 5'- cCAAgGcAAGCGACGACgGCcGCCG-Ca -3' miRNA: 3'- aGUUgCaUUCGCUGCUG-CGcUGGCuG- -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 30027 | 0.66 | 0.858766 |
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Target: 5'- gUCAACGUc-GCG--GACGUGaaGCCGACg -3' miRNA: 3'- -AGUUGCAuuCGCugCUGCGC--UGGCUG- -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 35084 | 0.66 | 0.866996 |
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Target: 5'- gUCGGCGaUGuuguguucuacGGCGACGuCGC-ACCGAUc -3' miRNA: 3'- -AGUUGC-AU-----------UCGCUGCuGCGcUGGCUG- -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 34437 | 0.66 | 0.874975 |
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Target: 5'- gUCGAcCGUGGagagcGCGAUGGCGUaguacGGCCGAg -3' miRNA: 3'- -AGUU-GCAUU-----CGCUGCUGCG-----CUGGCUg -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 26344 | 0.66 | 0.874975 |
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Target: 5'- gCGACGc--GCGcACGACGaCGGCCG-Cu -3' miRNA: 3'- aGUUGCauuCGC-UGCUGC-GCUGGCuG- -5' |
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| 12137 | 5' | -52.7 | NC_003309.1 | + | 11982 | 0.66 | 0.874975 |
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Target: 5'- --cGCGgcAGCcGCGGCGCGcGCCG-Cg -3' miRNA: 3'- aguUGCauUCGcUGCUGCGC-UGGCuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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