Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12138 | 5' | -53.1 | NC_003309.1 | + | 31999 | 0.66 | 0.831499 |
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Target: 5'- cCGCGGCGUcgcaCGUCAgcCGCaaacuGGCGCa -3' miRNA: 3'- -GCGCCGUAa---GUAGU--GCGcacu-UCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 37572 | 0.66 | 0.81286 |
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Target: 5'- cCGCGGCGUcaaCGUCGaa-GUGAaggggcccgGGCGCu -3' miRNA: 3'- -GCGCCGUAa--GUAGUgcgCACU---------UCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 3550 | 0.66 | 0.81286 |
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Target: 5'- gGCGGCGU--AUCGCGaCGacUGAucgccGCGCa -3' miRNA: 3'- gCGCCGUAagUAGUGC-GC--ACUu----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 47858 | 0.66 | 0.81286 |
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Target: 5'- aGCGcGC-UUCGUCACGuCGccuccUGAucGCGCg -3' miRNA: 3'- gCGC-CGuAAGUAGUGC-GC-----ACUu-CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 10610 | 0.66 | 0.81286 |
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Target: 5'- uGcCGGCAacguUCGCGCGUGccGuCGCg -3' miRNA: 3'- gC-GCCGUaaguAGUGCGCACuuC-GCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 8829 | 0.66 | 0.803242 |
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Target: 5'- cCGC-GCAUUCG-CuuGCGUGu-GCGCg -3' miRNA: 3'- -GCGcCGUAAGUaGugCGCACuuCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 33838 | 0.66 | 0.803242 |
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Target: 5'- aCGUGGCAUUCGUgAuCGaCGUGuu-CGCc -3' miRNA: 3'- -GCGCCGUAAGUAgU-GC-GCACuucGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 18842 | 0.66 | 0.8405 |
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Target: 5'- gCGCGGCAagCGUCAC-CGUGucacCGUu -3' miRNA: 3'- -GCGCCGUaaGUAGUGcGCACuuc-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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