Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12138 | 5' | -53.1 | NC_003309.1 | + | 37572 | 0.66 | 0.81286 |
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Target: 5'- cCGCGGCGUcaaCGUCGaa-GUGAaggggcccgGGCGCu -3' miRNA: 3'- -GCGCCGUAa--GUAGUgcgCACU---------UCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 43528 | 0.67 | 0.763037 |
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Target: 5'- cCGuCGGCGgcCAUCGCGCGcaucAGGuCGCg -3' miRNA: 3'- -GC-GCCGUaaGUAGUGCGCac--UUC-GCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 45881 | 0.67 | 0.793439 |
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Target: 5'- uCGCGcGCGUUgAUCACGCGgcGAcGGcCGUc -3' miRNA: 3'- -GCGC-CGUAAgUAGUGCGCa-CU-UC-GCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 46260 | 0.68 | 0.687819 |
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Target: 5'- aGCGGCAUcgAUCaACGCcUGAAccuucGCGCg -3' miRNA: 3'- gCGCCGUAagUAG-UGCGcACUU-----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 47858 | 0.66 | 0.81286 |
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Target: 5'- aGCGcGC-UUCGUCACGuCGccuccUGAucGCGCg -3' miRNA: 3'- gCGC-CGuAAGUAGUGC-GC-----ACUu-CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 47924 | 0.68 | 0.698824 |
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Target: 5'- -uCGGUcgUCGcgCACGCGUGccGUGCc -3' miRNA: 3'- gcGCCGuaAGUa-GUGCGCACuuCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 49887 | 0.68 | 0.720622 |
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Target: 5'- -cCGGCucaaGUUcCAUCGCGCGaauGAGCGCg -3' miRNA: 3'- gcGCCG----UAA-GUAGUGCGCac-UUCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 50830 | 0.66 | 0.8405 |
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Target: 5'- gGCGGUAUUacUAUCACaGgGUGAAuGaCGCc -3' miRNA: 3'- gCGCCGUAA--GUAGUG-CgCACUU-C-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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