Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12138 | 5' | -53.1 | NC_003309.1 | + | 11983 | 0.67 | 0.773325 |
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Target: 5'- gCGCGGCAgccgCggCGCGCGccgcgucggcguUGAAGC-Cg -3' miRNA: 3'- -GCGCCGUaa--GuaGUGCGC------------ACUUCGcG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 11547 | 0.77 | 0.24379 |
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Target: 5'- uGCGGCcacGUUCAUCGC-CGUGAGugcuuGCGCg -3' miRNA: 3'- gCGCCG---UAAGUAGUGcGCACUU-----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 10610 | 0.66 | 0.81286 |
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Target: 5'- uGcCGGCAacguUCGCGCGUGccGuCGCg -3' miRNA: 3'- gC-GCCGUaaguAGUGCGCACuuC-GCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 9709 | 0.7 | 0.56553 |
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Target: 5'- aGCGGUugccgugUGUCGCGCGcGAuGCGCa -3' miRNA: 3'- gCGCCGuaa----GUAGUGCGCaCUuCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 8829 | 0.66 | 0.803242 |
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Target: 5'- cCGC-GCAUUCG-CuuGCGUGu-GCGCg -3' miRNA: 3'- -GCGcCGUAAGUaGugCGCACuuCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 4988 | 0.68 | 0.709762 |
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Target: 5'- aGCGGC-UUC--CGCGCGUucGAucuGCGCg -3' miRNA: 3'- gCGCCGuAAGuaGUGCGCA--CUu--CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 3550 | 0.66 | 0.81286 |
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Target: 5'- gGCGGCGU--AUCGCGaCGacUGAucgccGCGCa -3' miRNA: 3'- gCGCCGUAagUAGUGC-GC--ACUu----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 3256 | 0.67 | 0.742058 |
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Target: 5'- aGCGGCcugaaCGUCAUGCccuccugcaGGAGCGCa -3' miRNA: 3'- gCGCCGuaa--GUAGUGCGca-------CUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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