Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12138 | 5' | -53.1 | NC_003309.1 | + | 9709 | 0.7 | 0.56553 |
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Target: 5'- aGCGGUugccgugUGUCGCGCGcGAuGCGCa -3' miRNA: 3'- gCGCCGuaa----GUAGUGCGCaCUuCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 16020 | 0.71 | 0.554563 |
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Target: 5'- gGCGGCGUagUCAUCGgaUGCG---AGCGCg -3' miRNA: 3'- gCGCCGUA--AGUAGU--GCGCacuUCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 22664 | 0.71 | 0.543659 |
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Target: 5'- gGCGGCGgcacCAUCGCccacgccgGCGgcgcugGAAGCGCc -3' miRNA: 3'- gCGCCGUaa--GUAGUG--------CGCa-----CUUCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 32981 | 0.72 | 0.490343 |
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Target: 5'- uCGCaGCccgCAcCGCGCGcUGAGGCGCg -3' miRNA: 3'- -GCGcCGuaaGUaGUGCGC-ACUUCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 17815 | 0.74 | 0.380144 |
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Target: 5'- gCGuCGGCGaUCGUCGCcgugugcgcguucgGCGUGAuacGGCGCa -3' miRNA: 3'- -GC-GCCGUaAGUAGUG--------------CGCACU---UCGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 11547 | 0.77 | 0.24379 |
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Target: 5'- uGCGGCcacGUUCAUCGC-CGUGAGugcuuGCGCg -3' miRNA: 3'- gCGCCG---UAAGUAGUGcGCACUU-----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 12058 | 0.79 | 0.181348 |
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Target: 5'- cCGCGGCGUUgGUggaUACGCGUGAcgucuGCGCc -3' miRNA: 3'- -GCGCCGUAAgUA---GUGCGCACUu----CGCG- -5' |
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| 12138 | 5' | -53.1 | NC_003309.1 | + | 21960 | 1.12 | 0.001016 |
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Target: 5'- gCGCGGCAUUCAUCACGCGUGAAGCGCu -3' miRNA: 3'- -GCGCCGUAAGUAGUGCGCACUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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