Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12158 | 5' | -56.4 | NC_003309.1 | + | 639 | 0.69 | 0.440299 |
Target: 5'- cGCGCGAAcuuGAGCcccUGCUCua--CGCGCGg -3' miRNA: 3'- -CGCGCUU---UUCG---ACGAGcgugGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 1990 | 0.72 | 0.310136 |
Target: 5'- gGUGCGAcAGGUUcgUUGCGCCGUGCGg -3' miRNA: 3'- -CGCGCUuUUCGAcgAGCGUGGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 3322 | 0.67 | 0.585459 |
Target: 5'- cGCGCGAugucGAGC-GCggaaaggCGCAgCGCGuCGa -3' miRNA: 3'- -CGCGCUu---UUCGaCGa------GCGUgGCGC-GC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 5181 | 0.74 | 0.237196 |
Target: 5'- cGCGCucgaucgccaguuucGAGgcGAGCUGCgCGuCGCCGCGCGc -3' miRNA: 3'- -CGCG---------------CUU--UUCGACGaGC-GUGGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 6140 | 0.67 | 0.563805 |
Target: 5'- cGCGCGAuu-GCaGCuUCGCuuaCGCGCc -3' miRNA: 3'- -CGCGCUuuuCGaCG-AGCGug-GCGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 7133 | 0.66 | 0.629163 |
Target: 5'- -aGCGGAugcGCaUGCUCGCgACCGuUGCa -3' miRNA: 3'- cgCGCUUuu-CG-ACGAGCG-UGGC-GCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 8810 | 0.68 | 0.531749 |
Target: 5'- uGCGCGuc--GUcGCUCGCA-CGCGUGa -3' miRNA: 3'- -CGCGCuuuuCGaCGAGCGUgGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 9522 | 0.66 | 0.640118 |
Target: 5'- cCGCGucGAGC-GCgCGCACCGUGa- -3' miRNA: 3'- cGCGCuuUUCGaCGaGCGUGGCGCgc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 9716 | 0.66 | 0.661998 |
Target: 5'- cGCGuCGAGcGGUUGC-CGUGugUCGCGCGc -3' miRNA: 3'- -CGC-GCUUuUCGACGaGCGU--GGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 10490 | 0.69 | 0.479922 |
Target: 5'- uCGCGA--GGCUuucgCGCAUCGCGCGc -3' miRNA: 3'- cGCGCUuuUCGAcga-GCGUGGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 10586 | 0.68 | 0.500377 |
Target: 5'- uCGCGAcauGC-GCUUGCGCCGcCGCc -3' miRNA: 3'- cGCGCUuuuCGaCGAGCGUGGC-GCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 10719 | 0.71 | 0.35847 |
Target: 5'- aGCGCGAgcGAAGCcucgGCgCGCucgagcuccuGCUGCGCGg -3' miRNA: 3'- -CGCGCU--UUUCGa---CGaGCG----------UGGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 10879 | 0.72 | 0.310136 |
Target: 5'- cGCGCGuuccGCUcGCUCGCGCgccUGCGUGg -3' miRNA: 3'- -CGCGCuuuuCGA-CGAGCGUG---GCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 10950 | 0.7 | 0.430686 |
Target: 5'- cGCGCGAGcgccGuCUGCgcCGuCGCCGCGCu -3' miRNA: 3'- -CGCGCUUuu--C-GACGa-GC-GUGGCGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 11076 | 0.69 | 0.459884 |
Target: 5'- uCGCGGAAcAGCaaccaGUUCGCgGCCGCGCc -3' miRNA: 3'- cGCGCUUU-UCGa----CGAGCG-UGGCGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 11234 | 0.7 | 0.430686 |
Target: 5'- aCGCGGAcuGC-GCgUCGUcuGCCGCGCGc -3' miRNA: 3'- cGCGCUUuuCGaCG-AGCG--UGGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 11508 | 0.67 | 0.596349 |
Target: 5'- cUGCGAcgcGAGGCUcGCcgugucgaUCGCGCCG-GCGg -3' miRNA: 3'- cGCGCU---UUUCGA-CG--------AGCGUGGCgCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 11984 | 0.75 | 0.217045 |
Target: 5'- cGCGCGGc-AGCcGCggcgCGCGCCGCGuCGg -3' miRNA: 3'- -CGCGCUuuUCGaCGa---GCGUGGCGC-GC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 12651 | 0.8 | 0.090028 |
Target: 5'- aUGCGAGccGCUGCUCGagauaGCCGCGCu -3' miRNA: 3'- cGCGCUUuuCGACGAGCg----UGGCGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 16126 | 0.73 | 0.260216 |
Target: 5'- uCGCGAugccGCUgcGCUCGCACauCGCGCGa -3' miRNA: 3'- cGCGCUuuu-CGA--CGAGCGUG--GCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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