Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12158 | 5' | -56.4 | NC_003309.1 | + | 11984 | 0.75 | 0.217045 |
Target: 5'- cGCGCGGc-AGCcGCggcgCGCGCCGCGuCGg -3' miRNA: 3'- -CGCGCUuuUCGaCGa---GCGUGGCGC-GC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 36315 | 0.76 | 0.17536 |
Target: 5'- aGCaGCaGAugaaGGAGCUGCUCGCACC-CGCa -3' miRNA: 3'- -CG-CG-CU----UUUCGACGAGCGUGGcGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 31664 | 0.77 | 0.140954 |
Target: 5'- cCGCGAcauuccGAAGCUGCUCGCAgugcccgcUCGCGUGc -3' miRNA: 3'- cGCGCU------UUUCGACGAGCGU--------GGCGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 46488 | 0.79 | 0.112834 |
Target: 5'- -gGCGGGAA-CUGCUCGCGCauaGCGCGg -3' miRNA: 3'- cgCGCUUUUcGACGAGCGUGg--CGCGC- -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 12651 | 0.8 | 0.090028 |
Target: 5'- aUGCGAGccGCUGCUCGagauaGCCGCGCu -3' miRNA: 3'- cGCGCUUuuCGACGAGCg----UGGCGCGc -5' |
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12158 | 5' | -56.4 | NC_003309.1 | + | 35681 | 1.11 | 0.000546 |
Target: 5'- uGCGCGAAAAGCUGCUCGCACCGCGCGa -3' miRNA: 3'- -CGCGCUUUUCGACGAGCGUGGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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