Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12171 | 5' | -61 | NC_003309.1 | + | 28479 | 0.66 | 0.400508 |
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Target: 5'- -cGCCAC-GCCGGCCCguauagcggcgaUUGCCCgaGGUUg -3' miRNA: 3'- cuUGGUGaUGGCCGGG------------GACGGG--UCGA- -5' |
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| 12171 | 5' | -61 | NC_003309.1 | + | 21824 | 0.67 | 0.317404 |
|
Target: 5'- aGGGCCGCUGuCgCGGCCCCUaCCacuccaauCAGCg -3' miRNA: 3'- -CUUGGUGAU-G-GCCGGGGAcGG--------GUCGa -5' |
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| 12171 | 5' | -61 | NC_003309.1 | + | 15270 | 0.68 | 0.302424 |
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Target: 5'- cGACCugUACuCGcGCCCCUGCCa---- -3' miRNA: 3'- cUUGGugAUG-GC-CGGGGACGGgucga -5' |
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| 12171 | 5' | -61 | NC_003309.1 | + | 25454 | 0.69 | 0.260771 |
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Target: 5'- -cACCGCcuucgcCCGGCCCCUGagugaCUCGGCUg -3' miRNA: 3'- cuUGGUGau----GGCCGGGGAC-----GGGUCGA- -5' |
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| 12171 | 5' | -61 | NC_003309.1 | + | 27376 | 0.71 | 0.16783 |
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Target: 5'- -cGCCACUcucGCCGGCCaccuaCUGCCC-GCc -3' miRNA: 3'- cuUGGUGA---UGGCCGGg----GACGGGuCGa -5' |
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| 12171 | 5' | -61 | NC_003309.1 | + | 42162 | 1.06 | 0.00035 |
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Target: 5'- aGAACCACUACCGGCCCCUGCCCAGCUc -3' miRNA: 3'- -CUUGGUGAUGGCCGGGGACGGGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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