miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12171 5' -61 NC_003309.1 + 42162 1.06 0.00035
Target:  5'- aGAACCACUACCGGCCCCUGCCCAGCUc -3'
miRNA:   3'- -CUUGGUGAUGGCCGGGGACGGGUCGA- -5'
12171 5' -61 NC_003309.1 + 27376 0.71 0.16783
Target:  5'- -cGCCACUcucGCCGGCCaccuaCUGCCC-GCc -3'
miRNA:   3'- cuUGGUGA---UGGCCGGg----GACGGGuCGa -5'
12171 5' -61 NC_003309.1 + 25454 0.69 0.260771
Target:  5'- -cACCGCcuucgcCCGGCCCCUGagugaCUCGGCUg -3'
miRNA:   3'- cuUGGUGau----GGCCGGGGAC-----GGGUCGA- -5'
12171 5' -61 NC_003309.1 + 15270 0.68 0.302424
Target:  5'- cGACCugUACuCGcGCCCCUGCCa---- -3'
miRNA:   3'- cUUGGugAUG-GC-CGGGGACGGgucga -5'
12171 5' -61 NC_003309.1 + 21824 0.67 0.317404
Target:  5'- aGGGCCGCUGuCgCGGCCCCUaCCacuccaauCAGCg -3'
miRNA:   3'- -CUUGGUGAU-G-GCCGGGGAcGG--------GUCGa -5'
12171 5' -61 NC_003309.1 + 28479 0.66 0.400508
Target:  5'- -cGCCAC-GCCGGCCCguauagcggcgaUUGCCCgaGGUUg -3'
miRNA:   3'- cuUGGUGaUGGCCGGG------------GACGGG--UCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.