Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12176 | 3' | -51.5 | NC_003309.1 | + | 9711 | 0.66 | 0.909897 |
Target: 5'- -cGAGCGGuugccguguGUCGcGCGCGAUG-CGCa -3' miRNA: 3'- uuCUUGCC---------UAGCuUGUGCUGCaGCGg -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 27340 | 0.66 | 0.909897 |
Target: 5'- cGGGAGCGGcgcggCGAACugcCGAUGcaucagacuggaUCGCCa -3' miRNA: 3'- -UUCUUGCCua---GCUUGu--GCUGC------------AGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 47871 | 0.66 | 0.902446 |
Target: 5'- -cGAGCGGccaUGaAGCGCGcuucgucACGUCGCCu -3' miRNA: 3'- uuCUUGCCua-GC-UUGUGC-------UGCAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 4185 | 0.66 | 0.901055 |
Target: 5'- cGAGGcagcGCGGAugucguccgccagcUCGAACGCGcCgGUCGCg -3' miRNA: 3'- -UUCU----UGCCU--------------AGCUUGUGCuG-CAGCGg -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 18706 | 0.66 | 0.896098 |
Target: 5'- ---uGCGGcgCGGAUACGGC--CGCCg -3' miRNA: 3'- uucuUGCCuaGCUUGUGCUGcaGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 17912 | 0.66 | 0.881191 |
Target: 5'- cGAGAACGaacgugcGUCGAACGucaugcCGACGagCGCCg -3' miRNA: 3'- -UUCUUGCc------UAGCUUGU------GCUGCa-GCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 13082 | 0.66 | 0.916372 |
Target: 5'- ---uGCGGcgCGGAcCACGAacUCGCCg -3' miRNA: 3'- uucuUGCCuaGCUU-GUGCUgcAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 19426 | 0.67 | 0.848241 |
Target: 5'- cGGGAACGcccauuucaGGUCGAucccguACACGAUGccCGCCg -3' miRNA: 3'- -UUCUUGC---------CUAGCU------UGUGCUGCa-GCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 15705 | 0.67 | 0.848241 |
Target: 5'- -cGAGCgcgGGAUCGcuCACGGgGUUGUCg -3' miRNA: 3'- uuCUUG---CCUAGCuuGUGCUgCAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 42658 | 0.68 | 0.791957 |
Target: 5'- -cGAACGGGccggCGAugGCGuccUGUCGUCg -3' miRNA: 3'- uuCUUGCCUa---GCUugUGCu--GCAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 24605 | 0.68 | 0.801835 |
Target: 5'- -cGAGCGGAUCGAcgAUGCGGCuaaucugcUCGUCu -3' miRNA: 3'- uuCUUGCCUAGCU--UGUGCUGc-------AGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 9540 | 0.68 | 0.801835 |
Target: 5'- ---cGCGGAUCGAACu--GCG-CGCCg -3' miRNA: 3'- uucuUGCCUAGCUUGugcUGCaGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 32013 | 0.68 | 0.811529 |
Target: 5'- gGGGAcCGGG-CGcacCGCGGCGUCGCa -3' miRNA: 3'- -UUCUuGCCUaGCuu-GUGCUGCAGCGg -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 29351 | 0.69 | 0.785946 |
Target: 5'- -cGAGCGGGcUGGAUAUGACGUgaaggcacuccaaauUGCCg -3' miRNA: 3'- uuCUUGCCUaGCUUGUGCUGCA---------------GCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 11476 | 0.69 | 0.771691 |
Target: 5'- --cGGCGGcUCGAacaaguucGCACGuaguuCGUCGCCg -3' miRNA: 3'- uucUUGCCuAGCU--------UGUGCu----GCAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 22638 | 0.69 | 0.771691 |
Target: 5'- cGGcGCuGGAagcgcCGGGCACGcCGUCGCCa -3' miRNA: 3'- uUCuUG-CCUa----GCUUGUGCuGCAGCGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 18331 | 0.69 | 0.76549 |
Target: 5'- uAGAcCGGAUCGGacggcauaucggccgACGCGACGacggaGCCg -3' miRNA: 3'- uUCUuGCCUAGCU---------------UGUGCUGCag---CGG- -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 22436 | 0.69 | 0.761327 |
Target: 5'- ----cCGGcGUCGAGCGCGGCG-CGCg -3' miRNA: 3'- uucuuGCC-UAGCUUGUGCUGCaGCGg -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 22467 | 0.69 | 0.740199 |
Target: 5'- --cGGCGGcAUCGuGCGCGGCGaUCGCa -3' miRNA: 3'- uucUUGCC-UAGCuUGUGCUGC-AGCGg -5' |
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12176 | 3' | -51.5 | NC_003309.1 | + | 37311 | 0.7 | 0.718618 |
Target: 5'- ---cGCGcGAUCGuGCGCGACGcccugaaucUCGCCg -3' miRNA: 3'- uucuUGC-CUAGCuUGUGCUGC---------AGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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