Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12179 | 3' | -57.1 | NC_003309.1 | + | 22583 | 0.69 | 0.404789 |
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Target: 5'- aGCGaCGCGCAgCCGCUcaGUGcCAGCGG-Ca -3' miRNA: 3'- -CGCaGUGCGU-GGCGG--UAC-GUCGUCaG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 22615 | 0.71 | 0.319467 |
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Target: 5'- cCGUCGC-CACCggcuGCCGUGCAGU-GUCg -3' miRNA: 3'- cGCAGUGcGUGG----CGGUACGUCGuCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 34211 | 0.7 | 0.349393 |
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Target: 5'- cCGUCGgGCuaccgaCGCCAUGCAGCAcaacuucgcgauccGUCg -3' miRNA: 3'- cGCAGUgCGug----GCGGUACGUCGU--------------CAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 43536 | 0.71 | 0.307134 |
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Target: 5'- cGCG-CACGCcgucgGCgGCCAUcgcgcgcaucaggucGCGGCAGUCu -3' miRNA: 3'- -CGCaGUGCG-----UGgCGGUA---------------CGUCGUCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 46161 | 1.12 | 0.000344 |
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Target: 5'- aGCGUCACGCACCGCCAUGCAGCAGUCg -3' miRNA: 3'- -CGCAGUGCGUGGCGGUACGUCGUCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 46837 | 0.67 | 0.53466 |
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Target: 5'- aCGgcaACGCGCCGUCA-GCGuCAGUCu -3' miRNA: 3'- cGCag-UGCGUGGCGGUaCGUcGUCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 48749 | 0.66 | 0.56686 |
|
Target: 5'- cGCcUCAUGCaACCGUCAUGCAagaGCgaaucaucaGGUCg -3' miRNA: 3'- -CGcAGUGCG-UGGCGGUACGU---CG---------UCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 51056 | 0.7 | 0.360371 |
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Target: 5'- gGCGUCaACGC-CCGCCAgaUGCcuGCGGa- -3' miRNA: 3'- -CGCAG-UGCGuGGCGGU--ACGu-CGUCag -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 53262 | 0.66 | 0.56686 |
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Target: 5'- cGC-UCGCGCGCCGUCuucgccGCGuGaCAGUCa -3' miRNA: 3'- -CGcAGUGCGUGGCGGua----CGU-C-GUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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