Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12179 | 3' | -57.1 | NC_003309.1 | + | 13446 | 0.67 | 0.492798 |
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Target: 5'- aGCGUCGuauCGCCGCCGUGCccuuGUAccGUCg -3' miRNA: 3'- -CGCAGUgc-GUGGCGGUACGu---CGU--CAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 9698 | 0.67 | 0.513554 |
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Target: 5'- uGUGUCGCGCGCgaUGC---GCAGCAcGUCg -3' miRNA: 3'- -CGCAGUGCGUG--GCGguaCGUCGU-CAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 10424 | 0.67 | 0.524067 |
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Target: 5'- cGCGUUuuGCAgcuUCGCCGcaGCAGCAGcUCg -3' miRNA: 3'- -CGCAGugCGU---GGCGGUa-CGUCGUC-AG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 46837 | 0.67 | 0.53466 |
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Target: 5'- aCGgcaACGCGCCGUCA-GCGuCAGUCu -3' miRNA: 3'- cGCag-UGCGUGGCGGUaCGUcGUCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 7808 | 0.66 | 0.556064 |
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Target: 5'- aGCGcCAUGUcgaGCgCGCCAUGUAcGCGcGUCa -3' miRNA: 3'- -CGCaGUGCG---UG-GCGGUACGU-CGU-CAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 53262 | 0.66 | 0.56686 |
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Target: 5'- cGC-UCGCGCGCCGUCuucgccGCGuGaCAGUCa -3' miRNA: 3'- -CGcAGUGCGUGGCGGua----CGU-C-GUCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 48749 | 0.66 | 0.56686 |
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Target: 5'- cGCcUCAUGCaACCGUCAUGCAagaGCgaaucaucaGGUCg -3' miRNA: 3'- -CGcAGUGCG-UGGCGGUACGU---CG---------UCAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 4146 | 0.66 | 0.577709 |
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Target: 5'- -gGUCGCGCucCCGCCGuUGCuGUcgauGUCg -3' miRNA: 3'- cgCAGUGCGu-GGCGGU-ACGuCGu---CAG- -5' |
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| 12179 | 3' | -57.1 | NC_003309.1 | + | 14819 | 0.66 | 0.588603 |
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Target: 5'- aCGUCACGCcCCGCUuaccGC-GCAGcCg -3' miRNA: 3'- cGCAGUGCGuGGCGGua--CGuCGUCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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