Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12188 | 3' | -50.8 | NC_003309.1 | + | 18712 | 0.66 | 0.909373 |
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Target: 5'- gCCGGcUGCGGcGcgGAuacGGCCgCCGa -3' miRNA: 3'- -GGCC-ACGCCuUuaCUuuuUCGG-GGCg -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 37573 | 0.67 | 0.8725 |
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Target: 5'- aCCGcG-GCGucaacgucGAAGUGAAGGGGCCCgggCGCu -3' miRNA: 3'- -GGC-CaCGC--------CUUUACUUUUUCGGG---GCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 37349 | 0.67 | 0.8725 |
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Target: 5'- aUCGGcGCGGAgucGAUGAGAucacGGGCgucuaCCUGCg -3' miRNA: 3'- -GGCCaCGCCU---UUACUUU----UUCG-----GGGCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 32253 | 0.68 | 0.864316 |
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Target: 5'- cCCGcG-GCGGAAagGAAGGGGCUaagaCCGUu -3' miRNA: 3'- -GGC-CaCGCCUUuaCUUUUUCGG----GGCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 23088 | 0.68 | 0.855878 |
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Target: 5'- cCUGGcugGCGGucgcAUGAAGuacagcgccGCCCCGCc -3' miRNA: 3'- -GGCCa--CGCCuu--UACUUUuu-------CGGGGCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 40506 | 0.68 | 0.847193 |
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Target: 5'- gCCGaUGCGGAcGUGGucuGGCCgCgGCg -3' miRNA: 3'- -GGCcACGCCUuUACUuuuUCGG-GgCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 45280 | 0.73 | 0.570985 |
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Target: 5'- gCCGGUacaGCGGAuuUGGucGAAugccGGCCgCCGCg -3' miRNA: 3'- -GGCCA---CGCCUuuACU--UUU----UCGG-GGCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 45206 | 0.76 | 0.433338 |
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Target: 5'- aUGGgGCGGuaauUGAAAAGGCCgCCGCc -3' miRNA: 3'- gGCCaCGCCuuu-ACUUUUUCGG-GGCG- -5' |
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| 12188 | 3' | -50.8 | NC_003309.1 | + | 52101 | 1.16 | 0.001122 |
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Target: 5'- cCCGGUGCGGAAAUGAAAAAGCCCCGCa -3' miRNA: 3'- -GGCCACGCCUUUACUUUUUCGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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