Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12219 | 3' | -53.8 | NC_003309.1 | + | 15037 | 0.65 | 0.817461 |
Target: 5'- gCCGA----CAGCGUucguguaacggaagGUCAGCgACGGCCg -3' miRNA: 3'- -GGCUcuacGUCGCG--------------UAGUUG-UGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 27341 | 0.66 | 0.810867 |
Target: 5'- aCGGGA-GCGGCGCggCGA-ACuGCCg -3' miRNA: 3'- gGCUCUaCGUCGCGuaGUUgUGcCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 11784 | 0.66 | 0.810867 |
Target: 5'- uUCGAGcgacuUGUA-CGCGUCGGCGCGcCCg -3' miRNA: 3'- -GGCUCu----ACGUcGCGUAGUUGUGCcGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 8745 | 0.66 | 0.801286 |
Target: 5'- cCCGGcGAUGUcccgaAGCGCuUCGAUccagaacauGCGGUCg -3' miRNA: 3'- -GGCU-CUACG-----UCGCGuAGUUG---------UGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 46660 | 0.66 | 0.801286 |
Target: 5'- aCCGGGc--CGGCGCGUaaugcgCAGCGCaccGGCCu -3' miRNA: 3'- -GGCUCuacGUCGCGUA------GUUGUG---CCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 18116 | 0.66 | 0.791526 |
Target: 5'- gUCGAccauCGaCGCAUCGACACGGUCg -3' miRNA: 3'- -GGCUcuacGUcGCGUAGUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 11988 | 0.66 | 0.781597 |
Target: 5'- cCCGcgc-GCGGCaGCcgCGGCGCGcGCCg -3' miRNA: 3'- -GGCucuaCGUCG-CGuaGUUGUGC-CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 46056 | 0.66 | 0.781597 |
Target: 5'- gUCGAGcAUGCgAGC-CAUCuuCuCGGCCc -3' miRNA: 3'- -GGCUC-UACG-UCGcGUAGuuGuGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 13489 | 0.66 | 0.771511 |
Target: 5'- cCCGcuGGGUGUAGC-CGUCGccgaAUugGGCUu -3' miRNA: 3'- -GGC--UCUACGUCGcGUAGU----UGugCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 1378 | 0.67 | 0.750914 |
Target: 5'- uUCGAGcAuuucgaucaccUGcCGGCGCuuGUCGaaGCACGGCCc -3' miRNA: 3'- -GGCUC-U-----------AC-GUCGCG--UAGU--UGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 7800 | 0.67 | 0.718053 |
Target: 5'- gUCGAGcgcgccAUGUAcGCGCGUCAcgacgaaAUACGGCg -3' miRNA: 3'- -GGCUC------UACGU-CGCGUAGU-------UGUGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 39605 | 0.67 | 0.719128 |
Target: 5'- gCGAGcGUGCuGCGCuUCGugACGGa- -3' miRNA: 3'- gGCUC-UACGuCGCGuAGUugUGCCgg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 23011 | 0.67 | 0.74987 |
Target: 5'- gCGGGAUGaugugguCAGCGaGUCGACGCuuGCCg -3' miRNA: 3'- gGCUCUAC-------GUCGCgUAGUUGUGc-CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 28364 | 0.67 | 0.750914 |
Target: 5'- -----cUGCAGCGC----ACGCGGCCa -3' miRNA: 3'- ggcucuACGUCGCGuaguUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 43529 | 0.67 | 0.750914 |
Target: 5'- gCCGucGGcgGCcaucgcGCGCAUCAGguCGCGGCa -3' miRNA: 3'- -GGC--UCuaCGu-----CGCGUAGUU--GUGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 14500 | 0.67 | 0.750914 |
Target: 5'- cCCGAcGuauUGCGGCGUAUCAAgcguCAuCGGUUg -3' miRNA: 3'- -GGCU-Cu--ACGUCGCGUAGUU----GU-GCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 41543 | 0.67 | 0.755075 |
Target: 5'- gCGAGAcaaucUGCGGCuccucgagacggaauGCggCAGCccuACGGCCg -3' miRNA: 3'- gGCUCU-----ACGUCG---------------CGuaGUUG---UGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 24050 | 0.67 | 0.76128 |
Target: 5'- aUCGAuaguUGCuuGCGUGcUCGACACGGCUc -3' miRNA: 3'- -GGCUcu--ACGu-CGCGU-AGUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 3944 | 0.68 | 0.686556 |
Target: 5'- uCCGAuGcgGCAGC-CAUCuGCGC-GCCg -3' miRNA: 3'- -GGCU-CuaCGUCGcGUAGuUGUGcCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 35600 | 0.68 | 0.653506 |
Target: 5'- aCGAgGAUGU-GCGCAUCGACA-GGUUc -3' miRNA: 3'- gGCU-CUACGuCGCGUAGUUGUgCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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