Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12219 | 3' | -53.8 | NC_003309.1 | + | 35484 | 0.77 | 0.245431 |
Target: 5'- gCCGAu-UGCAGCGCAUgGACACcgacGCCg -3' miRNA: 3'- -GGCUcuACGUCGCGUAgUUGUGc---CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 16008 | 0.76 | 0.251902 |
Target: 5'- aUCG-GAUGCgAGCGCgaauugguccGUCGGCGCGGCUg -3' miRNA: 3'- -GGCuCUACG-UCGCG----------UAGUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 18712 | 0.76 | 0.272156 |
Target: 5'- gCCG-GcUGCGGCGCG--GAUACGGCCg -3' miRNA: 3'- -GGCuCuACGUCGCGUagUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 43094 | 0.75 | 0.324457 |
Target: 5'- aCCGAGAcGCGGCGCGagCGACucACuGCCg -3' miRNA: 3'- -GGCUCUaCGUCGCGUa-GUUG--UGcCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 22398 | 0.73 | 0.382981 |
Target: 5'- uUCGGcAUGCcgcuGCGCGUCGaacgcccGCGCGGCCu -3' miRNA: 3'- -GGCUcUACGu---CGCGUAGU-------UGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 5164 | 0.73 | 0.383879 |
Target: 5'- uUCGAGGcgaGCuGCGCGUCGcCGCGcGCCg -3' miRNA: 3'- -GGCUCUa--CGuCGCGUAGUuGUGC-CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 5335 | 0.72 | 0.430522 |
Target: 5'- uUCGAGAgcgGCAGCGU----GCGCGcGCCg -3' miRNA: 3'- -GGCUCUa--CGUCGCGuaguUGUGC-CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 22474 | 0.71 | 0.501026 |
Target: 5'- gCCGAc--GCGGCgGCAUCGuGCGCGGCg -3' miRNA: 3'- -GGCUcuaCGUCG-CGUAGU-UGUGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 39001 | 0.71 | 0.522109 |
Target: 5'- uCCGGcgccAUGCAGCGCAUUgAGC-CGGCa -3' miRNA: 3'- -GGCUc---UACGUCGCGUAG-UUGuGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 9764 | 0.71 | 0.529569 |
Target: 5'- cCCGAGAUGCGaCGCGUUguuugcgauccgcuGAUAcagcuCGGCCg -3' miRNA: 3'- -GGCUCUACGUcGCGUAG--------------UUGU-----GCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 35657 | 0.7 | 0.565223 |
Target: 5'- gCGAGAUcCAGCGCGaCGACcaaGGCUg -3' miRNA: 3'- gGCUCUAcGUCGCGUaGUUGug-CCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 47496 | 0.7 | 0.587139 |
Target: 5'- cUCGAG--GCGGCGCAgCGACACcuucGCCg -3' miRNA: 3'- -GGCUCuaCGUCGCGUaGUUGUGc---CGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 18415 | 0.69 | 0.619169 |
Target: 5'- aCGAGcgcgaccGUGUAGCGCGUCuugcgcuCGCuGCCg -3' miRNA: 3'- gGCUC-------UACGUCGCGUAGuu-----GUGcCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 46487 | 0.69 | 0.620276 |
Target: 5'- gCGGGAacUGCucGCGCAU-AGCGCGGUUg -3' miRNA: 3'- gGCUCU--ACGu-CGCGUAgUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 29731 | 0.69 | 0.624707 |
Target: 5'- uCCGAcugggccaauGAUGCAGUGCAUCGuCgaucuaugcuaucugACGGCa -3' miRNA: 3'- -GGCU----------CUACGUCGCGUAGUuG---------------UGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 35600 | 0.68 | 0.653506 |
Target: 5'- aCGAgGAUGU-GCGCAUCGACA-GGUUc -3' miRNA: 3'- gGCU-CUACGuCGCGUAGUUGUgCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 35374 | 0.68 | 0.653506 |
Target: 5'- cCCaAGGUGCGcCGCgAUCGcACACGGCa -3' miRNA: 3'- -GGcUCUACGUcGCG-UAGU-UGUGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 22338 | 0.68 | 0.672275 |
Target: 5'- gCCGAucaGGUGCucgacgcaAGCGCcggccgccaugccaAgCAGCGCGGCCa -3' miRNA: 3'- -GGCU---CUACG--------UCGCG--------------UaGUUGUGCCGG- -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 16798 | 0.68 | 0.675577 |
Target: 5'- gCCGAGAuUGCgccGGC-CGUUgAACACGGCg -3' miRNA: 3'- -GGCUCU-ACG---UCGcGUAG-UUGUGCCGg -5' |
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12219 | 3' | -53.8 | NC_003309.1 | + | 3944 | 0.68 | 0.686556 |
Target: 5'- uCCGAuGcgGCAGC-CAUCuGCGC-GCCg -3' miRNA: 3'- -GGCU-CuaCGUCGcGUAGuUGUGcCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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