miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12255 3' -51.2 NC_003309.1 + 48020 0.66 0.923479
Target:  5'- ------cGGUCGAggUCGCCGGAuGCGa -3'
miRNA:   3'- agaauuuCUAGCUa-GGCGGCCU-CGCc -5'
12255 3' -51.2 NC_003309.1 + 15992 0.66 0.917339
Target:  5'- -------aAUUGGUCCGUCGGcGCGGc -3'
miRNA:   3'- agaauuucUAGCUAGGCGGCCuCGCC- -5'
12255 3' -51.2 NC_003309.1 + 53317 0.66 0.904202
Target:  5'- ------cGG-CGcAUCCGCCGG-GCGGg -3'
miRNA:   3'- agaauuuCUaGC-UAGGCGGCCuCGCC- -5'
12255 3' -51.2 NC_003309.1 + 47539 0.67 0.8824
Target:  5'- -------cGUCGAugcagUCCGCCGGGGCa- -3'
miRNA:   3'- agaauuucUAGCU-----AGGCGGCCUCGcc -5'
12255 3' -51.2 NC_003309.1 + 16171 0.69 0.803432
Target:  5'- ------cGAUCGAUCCGUCGGccugcacgccgAGCGa -3'
miRNA:   3'- agaauuuCUAGCUAGGCGGCC-----------UCGCc -5'
12255 3' -51.2 NC_003309.1 + 8557 0.71 0.698562
Target:  5'- aUCUgc--GAUCGGggaguuacgCCGCCGGcGGCGGc -3'
miRNA:   3'- -AGAauuuCUAGCUa--------GGCGGCC-UCGCC- -5'
12255 3' -51.2 NC_003309.1 + 28249 0.73 0.54274
Target:  5'- cUCUUccauAGGUCGAUCagaccaaGCCGGAGCu- -3'
miRNA:   3'- -AGAAuu--UCUAGCUAGg------CGGCCUCGcc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.