Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12255 | 5' | -51.3 | NC_003309.1 | + | 525 | 0.66 | 0.893476 |
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Target: 5'- aGGC-GCUGCCGgcucgccGGGUCGAGCc---- -3' miRNA: 3'- -UCGcCGACGGUa------CCUAGCUUGaaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 9558 | 0.66 | 0.869921 |
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Target: 5'- cGGCGGCuugUGCgUAUucgcGGAUCGAACUg--- -3' miRNA: 3'- -UCGCCG---ACG-GUA----CCUAGCUUGAaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 764 | 0.66 | 0.861525 |
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Target: 5'- cAGCGGC--CUccGGAUCGAACUUc-- -3' miRNA: 3'- -UCGCCGacGGuaCCUAGCUUGAAauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 16021 | 0.67 | 0.85287 |
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Target: 5'- cGGCGGCguaGUCAUcGGAUgCGAGCg---- -3' miRNA: 3'- -UCGCCGa--CGGUA-CCUA-GCUUGaaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 44474 | 0.67 | 0.85287 |
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Target: 5'- uGCcGCUGCCGaagaacGGAUCGAACa---- -3' miRNA: 3'- uCGcCGACGGUa-----CCUAGCUUGaaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 48594 | 0.67 | 0.825438 |
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Target: 5'- cAGCGGUaUGUCGUGGGgcgCGAGCc---- -3' miRNA: 3'- -UCGCCG-ACGGUACCUa--GCUUGaaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 22606 | 0.68 | 0.764947 |
|
Target: 5'- -cCGGCUGCCGUGcaguGUCGGACa---- -3' miRNA: 3'- ucGCCGACGGUACc---UAGCUUGaaauc -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 52578 | 0.68 | 0.764947 |
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Target: 5'- aGGaCGGCcaccgggccuuUGCCGUGGuccccAUCGAACUgUGGg -3' miRNA: 3'- -UC-GCCG-----------ACGGUACC-----UAGCUUGAaAUC- -5' |
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| 12255 | 5' | -51.3 | NC_003309.1 | + | 9710 | 0.71 | 0.596469 |
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Target: 5'- gAGCGGUUGCCGUGuGUCGcGCg---- -3' miRNA: 3'- -UCGCCGACGGUACcUAGCuUGaaauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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