Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12258 | 5' | -56.8 | NC_003309.1 | + | 17747 | 0.66 | 0.625118 |
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Target: 5'- cGCGCGCgGCa-GC-UCGAUCCGAu-- -3' miRNA: 3'- -CGCGCGgCGccCGcAGUUAGGCUuuc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 37580 | 0.67 | 0.592668 |
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Target: 5'- gGCGaCGaCCGC-GGCGUCAAcgUCGAAGu -3' miRNA: 3'- -CGC-GC-GGCGcCCGCAGUUa-GGCUUUc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 46831 | 0.67 | 0.592668 |
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Target: 5'- aCGCGCCGUcaGCGUCAGUCUu---- -3' miRNA: 3'- cGCGCGGCGccCGCAGUUAGGcuuuc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 8911 | 0.68 | 0.518435 |
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Target: 5'- cCGCGaCCGCGGucgcauCGUCG-UCCGAGAa -3' miRNA: 3'- cGCGC-GGCGCCc-----GCAGUuAGGCUUUc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 27741 | 0.69 | 0.457891 |
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Target: 5'- aCGCGCCGCccGGCG-CAucgCCGAAAu -3' miRNA: 3'- cGCGCGGCGc-CCGCaGUua-GGCUUUc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 25381 | 0.71 | 0.34943 |
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Target: 5'- uGCGUguaGCCGUcGGCGUCGGUCUGGccGg -3' miRNA: 3'- -CGCG---CGGCGcCCGCAGUUAGGCUuuC- -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 46738 | 0.72 | 0.302486 |
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Target: 5'- cGCGCGCCGgaaCGGGCucuuugauGUCGGUCgGAGu- -3' miRNA: 3'- -CGCGCGGC---GCCCG--------CAGUUAGgCUUuc -5' |
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| 12258 | 5' | -56.8 | NC_003309.1 | + | 11968 | 0.8 | 0.099209 |
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Target: 5'- cGCGCGCCGCGucGGCGUUGAagCCGAAc- -3' miRNA: 3'- -CGCGCGGCGC--CCGCAGUUa-GGCUUuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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