Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12268 | 3' | -55.4 | NC_003309.1 | + | 4662 | 0.71 | 0.39819 |
Target: 5'- ----gGCGCGCauucGCGCcuuGACGAUCGGCUc -3' miRNA: 3'- cuuagUGCGCG----CGCG---CUGCUAGUCGA- -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 45882 | 0.71 | 0.379202 |
Target: 5'- --cUCGCGCGCGUugaucacGCGGCGA-CGGCc -3' miRNA: 3'- cuuAGUGCGCGCG-------CGCUGCUaGUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 17533 | 0.71 | 0.379202 |
Target: 5'- uGGAUCugGCGCacccacgGCGCGuCGGacgUCAGCg -3' miRNA: 3'- -CUUAGugCGCG-------CGCGCuGCU---AGUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 5563 | 0.71 | 0.39819 |
Target: 5'- --uUCGCGCGCGCcgaucGCGGCGGUgAGg- -3' miRNA: 3'- cuuAGUGCGCGCG-----CGCUGCUAgUCga -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 13639 | 0.71 | 0.407444 |
Target: 5'- aGAGUC-CGCGgGCGCGaaGCGG-CGGCg -3' miRNA: 3'- -CUUAGuGCGCgCGCGC--UGCUaGUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 3302 | 0.71 | 0.407444 |
Target: 5'- ----aAgGCGCaGCGCGuCGAUCAGCg -3' miRNA: 3'- cuuagUgCGCG-CGCGCuGCUAGUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 16925 | 0.72 | 0.371252 |
Target: 5'- cGAUCGCGC-CGacaGCGACGAgugCGGCg -3' miRNA: 3'- cUUAGUGCGcGCg--CGCUGCUa--GUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 50240 | 0.72 | 0.362554 |
Target: 5'- -cGUCGCGCGCGCGUGAuucacauacagcCGAgcaAGCa -3' miRNA: 3'- cuUAGUGCGCGCGCGCU------------GCUag-UCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 34504 | 0.73 | 0.305696 |
Target: 5'- cGAAUCGauUGCGC-CGAUGAUCGGCUg -3' miRNA: 3'- -CUUAGUgcGCGCGcGCUGCUAGUCGA- -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 11767 | 0.75 | 0.230587 |
Target: 5'- -cGUCG-GCGCGCcCGGCGAUCAGCc -3' miRNA: 3'- cuUAGUgCGCGCGcGCUGCUAGUCGa -5' |
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12268 | 3' | -55.4 | NC_003309.1 | + | 23976 | 0.76 | 0.196574 |
Target: 5'- ----aACGCGC-CGCGACGAUCGGUg -3' miRNA: 3'- cuuagUGCGCGcGCGCUGCUAGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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