Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12268 | 3' | -55.4 | NC_003309.1 | + | 9651 | 0.68 | 0.571085 |
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Target: 5'- cGAUCugGUcaCGCGUGACGAUCcgaaGGCg -3' miRNA: 3'- cUUAGugCGc-GCGCGCUGCUAG----UCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 43188 | 0.67 | 0.604014 |
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Target: 5'- uGGUCACGUccGuCGCGCGAacCGcGUCAGCg -3' miRNA: 3'- cUUAGUGCG--C-GCGCGCU--GC-UAGUCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 10864 | 0.67 | 0.604014 |
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Target: 5'- --cUCGCGCGCcugcguggcaaGCGCGAC-AUCAcGCUc -3' miRNA: 3'- cuuAGUGCGCG-----------CGCGCUGcUAGU-CGA- -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 8545 | 0.67 | 0.626103 |
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Target: 5'- gGAGUUACGC-CGCcgGCGGCGGcacgagcuUCGGCg -3' miRNA: 3'- -CUUAGUGCGcGCG--CGCUGCU--------AGUCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 21880 | 0.67 | 0.63716 |
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Target: 5'- uGGAUCugGC-CGCGCGACa---AGCa -3' miRNA: 3'- -CUUAGugCGcGCGCGCUGcuagUCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 37331 | 0.67 | 0.648213 |
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Target: 5'- aGAUCACGgGCgucuaccuGCGCG-CGAUCGuGCg -3' miRNA: 3'- cUUAGUGCgCG--------CGCGCuGCUAGU-CGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 16539 | 0.66 | 0.659251 |
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Target: 5'- ---aCAC-CGCGCGCGuCGAUaGGCg -3' miRNA: 3'- cuuaGUGcGCGCGCGCuGCUAgUCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 36357 | 0.66 | 0.68124 |
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Target: 5'- aGGUCgACGCGCaguugaaCGCGACGAUCGaaacGCa -3' miRNA: 3'- cUUAG-UGCGCGc------GCGCUGCUAGU----CGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 10474 | 0.66 | 0.692169 |
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Target: 5'- -cAUCGCGCGCaUGCcGCGAguUCGGCc -3' miRNA: 3'- cuUAGUGCGCGcGCGcUGCU--AGUCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 31706 | 0.66 | 0.70304 |
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Target: 5'- -uGUCugGCuCgGCGCGACGAUC-GUg -3' miRNA: 3'- cuUAGugCGcG-CGCGCUGCUAGuCGa -5' |
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| 12268 | 3' | -55.4 | NC_003309.1 | + | 20302 | 0.66 | 0.70304 |
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Target: 5'- cGAUCAuCGCGUuCGUGAuccagcccgcacCGAUCAGCg -3' miRNA: 3'- cUUAGU-GCGCGcGCGCU------------GCUAGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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