Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12268 | 5' | -56.5 | NC_003309.1 | + | 30265 | 0.66 | 0.63716 |
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Target: 5'- -cGGCGCcuGGUaUUGGaGCGAGCCAg- -3' miRNA: 3'- gcUCGCG--UCAaAGCCgCGCUCGGUau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 4068 | 0.66 | 0.626103 |
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Target: 5'- aGAGCGCAGgccUUCGuaGCuGGUCAUc -3' miRNA: 3'- gCUCGCGUCa--AAGCcgCGcUCGGUAu -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 25863 | 0.67 | 0.571085 |
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Target: 5'- gGGGCGCcgaacAGcacCGGCGCGAGCgGg- -3' miRNA: 3'- gCUCGCG-----UCaaaGCCGCGCUCGgUau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 5621 | 0.67 | 0.549369 |
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Target: 5'- aGaAGCGCAGgcccUUCGGgGUGuucGCCGUGc -3' miRNA: 3'- gC-UCGCGUCa---AAGCCgCGCu--CGGUAU- -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 48602 | 0.67 | 0.538607 |
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Target: 5'- uGAGCaagcaGCGGUaugUCguggGGCGCGAGCCu-- -3' miRNA: 3'- gCUCG-----CGUCAa--AG----CCGCGCUCGGuau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 11987 | 0.68 | 0.496351 |
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Target: 5'- cCGcGCGCGGcagccgCGGCGCGcGCCGc- -3' miRNA: 3'- -GCuCGCGUCaaa---GCCGCGCuCGGUau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 49863 | 0.69 | 0.405582 |
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Target: 5'- uGAGCGCGGUucggagcacguucUUCGcGCGCGGcucgcucGCCAg- -3' miRNA: 3'- gCUCGCGUCA-------------AAGC-CGCGCU-------CGGUau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 16001 | 0.7 | 0.39819 |
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Target: 5'- gCGAGCGCgaauuGGUccgUCGGCGCG-GCUg-- -3' miRNA: 3'- -GCUCGCG-----UCAa--AGCCGCGCuCGGuau -5' |
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| 12268 | 5' | -56.5 | NC_003309.1 | + | 19299 | 0.71 | 0.329193 |
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Target: 5'- cCGAGCGCGuUUUCGGCGCGcacGCgCGc- -3' miRNA: 3'- -GCUCGCGUcAAAGCCGCGCu--CG-GUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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