Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12329 | 3' | -55.6 | NC_003324.1 | + | 53668 | 0.7 | 0.477707 |
Target: 5'- aGUGUCAUGUCGCaaaGGGGGAccGGCaucagGCGg -3' miRNA: 3'- aCGCGGUAUAGCGg--CCUCUU--UCG-----CGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 51304 | 0.71 | 0.400355 |
Target: 5'- cGCGgaCCAUcgCGCCGGAGuAAAGCa-- -3' miRNA: 3'- aCGC--GGUAuaGCGGCCUC-UUUCGcgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 50281 | 0.71 | 0.409585 |
Target: 5'- aUGCGCCGccgGUCGaCGGcaAGAAGGCGUu -3' miRNA: 3'- -ACGCGGUa--UAGCgGCC--UCUUUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 50274 | 0.66 | 0.682088 |
Target: 5'- -uCGCCGacuUGUCGCUgaugcccauGGcgaAGAAAGCGCGg -3' miRNA: 3'- acGCGGU---AUAGCGG---------CC---UCUUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 48444 | 0.67 | 0.649262 |
Target: 5'- aGCGCCg---CGCCGaGAaGAuGGUGCGc -3' miRNA: 3'- aCGCGGuauaGCGGC-CU-CUuUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 47706 | 0.66 | 0.725137 |
Target: 5'- gGCgGCCcgGUCGCCGucau--GCGCGg -3' miRNA: 3'- aCG-CGGuaUAGCGGCcucuuuCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 43795 | 0.71 | 0.428438 |
Target: 5'- gGUGCCGggaaCGCCGGc-AAGGCGCGu -3' miRNA: 3'- aCGCGGUaua-GCGGCCucUUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 42419 | 0.67 | 0.616293 |
Target: 5'- aGCGCCAg--CGCCaGAcaGGAAGCGa- -3' miRNA: 3'- aCGCGGUauaGCGGcCU--CUUUCGCgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 42316 | 0.66 | 0.682088 |
Target: 5'- cGUGCCGgg-CGCCGGuGAcagacuuaGAGCGa- -3' miRNA: 3'- aCGCGGUauaGCGGCCuCU--------UUCGCgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 41130 | 0.7 | 0.476694 |
Target: 5'- cGCGCUGcaaggcUAUCGCCGGcucacacucgaacAGcGAGGCGCGu -3' miRNA: 3'- aCGCGGU------AUAGCGGCC-------------UC-UUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38635 | 0.66 | 0.692949 |
Target: 5'- cGUGCCcUAUCGCCcGGAGcu-GC-CGg -3' miRNA: 3'- aCGCGGuAUAGCGG-CCUCuuuCGcGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38368 | 0.7 | 0.457651 |
Target: 5'- gGuCGCCGUcgUGCgGGAGGAAGUGg- -3' miRNA: 3'- aC-GCGGUAuaGCGgCCUCUUUCGCgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38247 | 0.71 | 0.409585 |
Target: 5'- aGCGCCGgaaugccgAUCGCCaucaggucgGGAGu--GCGCGg -3' miRNA: 3'- aCGCGGUa-------UAGCGG---------CCUCuuuCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38164 | 0.7 | 0.477707 |
Target: 5'- gGCGCCGacgcgcuUCGCC--AGAAGGCGCa -3' miRNA: 3'- aCGCGGUau-----AGCGGccUCUUUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 37994 | 0.67 | 0.623985 |
Target: 5'- gGCgGCCA---UGCCGGAGcgguugcggcgcacGGGGCGCGu -3' miRNA: 3'- aCG-CGGUauaGCGGCCUC--------------UUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 36723 | 0.67 | 0.645967 |
Target: 5'- gGCGgCAccuUCGCCGGGGAAaucuaugacccgcuAucGCGCGg -3' miRNA: 3'- aCGCgGUau-AGCGGCCUCUU--------------U--CGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 35127 | 0.67 | 0.660233 |
Target: 5'- aGCGCC--GUCGCCGuuGAccucAGCGCc -3' miRNA: 3'- aCGCGGuaUAGCGGCcuCUu---UCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 34224 | 0.66 | 0.714485 |
Target: 5'- uUGUGCCGgucccugaccUGUCaCCGGucgacaGGGAGGCGUGa -3' miRNA: 3'- -ACGCGGU----------AUAGcGGCC------UCUUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 33460 | 0.7 | 0.447793 |
Target: 5'- -cCGCCA-GUgGCCGGAGuu-GCGCGc -3' miRNA: 3'- acGCGGUaUAgCGGCCUCuuuCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 32484 | 0.66 | 0.703752 |
Target: 5'- gUGCGCgGUAagagcgCGCCG-AGguGGUGCGa -3' miRNA: 3'- -ACGCGgUAUa-----GCGGCcUCuuUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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