Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12332 | 3' | -57.7 | NC_003324.1 | + | 14572 | 1.08 | 0.000632 |
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Target: 5'- cAGUGUUGGCUGCACCGGCUUCCGCGAc -3' miRNA: 3'- -UCACAACCGACGUGGCCGAAGGCGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 5737 | 0.75 | 0.160243 |
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Target: 5'- --cGUUGGCgGCACCGGCUaCCGUu- -3' miRNA: 3'- ucaCAACCGaCGUGGCCGAaGGCGcu -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 4220 | 0.71 | 0.299006 |
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Target: 5'- aGGUGaUGGUguCGCCGGCUUCCGauuCGAg -3' miRNA: 3'- -UCACaACCGacGUGGCCGAAGGC---GCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 14656 | 0.69 | 0.379711 |
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Target: 5'- -----cGGCcgcuUGCGCCGGCaggCCGCGAc -3' miRNA: 3'- ucacaaCCG----ACGUGGCCGaa-GGCGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 42867 | 0.69 | 0.388543 |
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Target: 5'- -----aGGCUGCcgGCCGGC--CCGCGAg -3' miRNA: 3'- ucacaaCCGACG--UGGCCGaaGGCGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 19003 | 0.69 | 0.397509 |
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Target: 5'- --cGgcGGCUGCACgCGGCUggccucgaaCGCGAu -3' miRNA: 3'- ucaCaaCCGACGUG-GCCGAag-------GCGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 35315 | 0.68 | 0.444268 |
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Target: 5'- --cGgcGGaUUGCGCCGGCUgCUGCGGu -3' miRNA: 3'- ucaCaaCC-GACGUGGCCGAaGGCGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 30081 | 0.67 | 0.535461 |
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Target: 5'- -----cGGCcGCugCGGCUUCCuCGAc -3' miRNA: 3'- ucacaaCCGaCGugGCCGAAGGcGCU- -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 33359 | 0.66 | 0.546035 |
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Target: 5'- cGGUGcgUGGUU--GCCGGCU-CCGCGc -3' miRNA: 3'- -UCACa-ACCGAcgUGGCCGAaGGCGCu -5' |
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| 12332 | 3' | -57.7 | NC_003324.1 | + | 44402 | 0.66 | 0.599743 |
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Target: 5'- ----gUGGCUGCAgaGGCUaucggCCGCGc -3' miRNA: 3'- ucacaACCGACGUggCCGAa----GGCGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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